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Catalog Number Size Price
LS-C662257-10 10 µg $318 
LS-C662257-100 100 µg $470 
BAG5 Antibody - Western blot analysis of Bag5 using anti-Bag5 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: HELA whole Cell lysates, Lane 4: MCF-7 whole cell lysates, Lane 5: SKOV3 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bag5 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Bag5 at approximately 51KD. The expected band size for Bag5 is at 51KD.

Polyclonal Rabbit anti‑Human BAG5 Antibody (WB) LS‑C662257

Polyclonal Rabbit anti‑Human BAG5 Antibody (WB) LS‑C662257

Antibody:
BAG5 Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662257-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
BAG5 Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
BAG5 antibody LS-C662257 is an unconjugated rabbit polyclonal antibody to human BAG5. Validated for WB.
Target
Human BAG5
Synonyms
BAG5 | BAG-5 | KIAA0873 | Bcl-2-associated athanogene 5 | BCL2-associated athanogene 5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human Bag5 recombinant protein (Position: N389-Y447). Human Bag5 shares 96.6% and 93.2% amino acid (aa) sequence identity with mouse and rat Bag5, respectively.
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BAG5
Q9UL15 NM_004873 BAA74896.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/18/2024