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Catalog Number Size Price
LS-C662152-10 10 µg $318 
LS-C662152-100 100 µg $470 
BAG1 / BAG-1 Antibody - Immunohistochemistry - Anti-Bag1 Picoband Antibody
BAG1 / BAG-1 Antibody - Immunohistochemistry - Anti-Bag1 Picoband Antibody
BAG1 / BAG-1 Antibody - Immunohistochemistry - Anti-Bag1 Picoband Antibody
BAG1 / BAG-1 Antibody - Western blot analysis of Bag1 using anti-Bag1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bag1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Bag1 at approximately 39KD. The expected band size for Bag1 is at 39KD.
BAG1 / BAG-1 Antibody - Western blot - Anti-Bag1 Picoband Antibody
BAG1 / BAG-1 Antibody - Immunohistochemistry - Anti-Bag1 Picoband Antibody
BAG1 / BAG-1 Antibody - Immunohistochemistry - Anti-Bag1 Picoband Antibody
BAG1 / BAG-1 Antibody - Immunohistochemistry - Anti-Bag1 Picoband Antibody
BAG1 / BAG-1 Antibody - Western blot analysis of Bag1 using anti-Bag1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bag1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Bag1 at approximately 39KD. The expected band size for Bag1 is at 39KD.
BAG1 / BAG-1 Antibody - Western blot - Anti-Bag1 Picoband Antibody
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Polyclonal Rabbit anti‑Human BAG1 / BAG‑1 Antibody (IHC, WB) LS‑C662152

Polyclonal Rabbit anti‑Human BAG1 / BAG‑1 Antibody (IHC, WB) LS‑C662152

Antibody:
BAG1 / BAG-1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662152-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
BAG1 / BAG-1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
BAG-1 antibody LS-C662152 is an unconjugated rabbit polyclonal antibody to human BAG-1 (BAG1). Validated for IHC and WB.
Target
Human BAG1 / BAG-1
Synonyms
BAG1 | Bcl-2-associated athanogene 1 | Bcl-2-binding protein | Bcl2 binding protein bag1 | BCL2-associated athanogene | BAG-1 | Bcl2 binding athanogene1 | HAP | RAP46
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human Bag1 (323-345 aa DTVEQNICQETERLQSTNFALAE), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.
Specificity
Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level). .
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BAG1 / BAG-1
NM_004323

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/29/2024