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Antibodies > ATXN1 / SCA1 > LS-C801117
 
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Catalog Number Size Price
LS-C801117-100 100 µl (1 mg/ml) $379 
LS-C801117-200 200 µl (1 mg/ml) $421 
ATXN1 / SCA1 Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
ATXN1 / SCA1 Antibody - Staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
ATXN1 / SCA1 Antibody - Western blot analysis of Ataxin 1 phosphorylation expression in Adriamycin treated HepG2 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
ATXN1 / SCA1 Antibody - Western blot analysis of Phospho-Ataxin 1 (Ser775) expression in various lysates
ATXN1 / SCA1 Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
ATXN1 / SCA1 Antibody - Staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
ATXN1 / SCA1 Antibody - Western blot analysis of Ataxin 1 phosphorylation expression in Adriamycin treated HepG2 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
ATXN1 / SCA1 Antibody - Western blot analysis of Phospho-Ataxin 1 (Ser775) expression in various lysates
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Polyclonal Rabbit anti‑Human ATXN1 / SCA1 Antibody (phospho‑Ser775, IF, WB) LS‑C801117

Polyclonal Rabbit anti‑Human ATXN1 / SCA1 Antibody (phospho‑Ser775, IF, WB) LS‑C801117

Submit Review LSBio Guarantee
Antibody:
ATXN1 / SCA1 Rabbit anti-Human Polyclonal (pSer775) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801117-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ATXN1 / SCA1 Rabbit anti-Human Polyclonal (pSer775) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
SCA1 antibody LS-C801117 is an unconjugated rabbit polyclonal antibody to SCA1 (ATXN1) (pSer775) from human. It is reactive with human and mouse. Validated for IF, Peptide-ELISA and WB.
Target
Human ATXN1 / SCA1
Synonyms
ATXN1 | ATX1 | Ataxin 1 | D6S504E | Ataxin-1 | SCA1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Ataxin 1 around the phosphorylation site of Serine 775.
Epitope
pSer775
Specificity
Phospho-Ataxin 1 (Ser775) Antibody detects endogenous levels of Ataxin 1 only when phosphorylated at Serine 775.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ATXN1 / SCA1
P54253 NM_000332 NP_000323.2

Publications (0)

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ATXN1 / SCA1 Antibody - Immunohistochemical analysis of Ataxin 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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ATXN1 / SCA1 Antibody - Western blot of Ataxin-1 antibody
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ATXN1 / SCA1 Antibody - Western blot of Ataxin 1 expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
ATXN1 / SCA1 Antibody LS‑C417564
Species: Human, Mouse
Applications: IHC, ICC, Immunofluorescence, Western blot
See all ATXN1 / SCA1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
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