Target
Rat ATP1A1
Synonyms
ATP1A1 | Na+, K+ ATPase alpha subunit | Na,K-ATPase alpha-1 subunit | Na+/K+ ATPase 1 | Sodium pump 1 | Sodium pump subunit alpha-1
Reactivity
Rat, Bovine
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
Fusion protein containing residues 338-518 of the a-1 subunit, as deduced from the cDNA.
Specificity
Specific for the a-1 subunit isoform at Mr of 110kD. Species cross-reactivity: Rat and bovine. Does not cross-react with rabbit.
Applications
- IHC
- IHC - Frozen
- ICC (5 µg/ml)
- Western blot (0.5 - 2 µg/ml)
- Immunoprecipitation
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Usage
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Previous lots were successfully used to immunoprecipitate the a-1 subunit from of rat brain microsomes. Immunocytochemistry: 5 ug/ml detects the Na+ /K+ ATPase a-1 subunit in frozen sections of rat kidney and brain, which were fixed in 4% paraformaldehyde.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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