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Catalog Number Size Price
LS-C776182-100 100 µg (1 mg/ml) $473 
ATG4D Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4D Polyclonal Antibody. Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4D Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4D Antibody (D) Composite.
ATG4D Antibody - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~52.9kDa ATG4D protein using Rabbit Anti-ATG4D Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG4D Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~52.9kDa.
ATG4D Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4D Polyclonal Antibody. Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4D Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4D Antibody (D) Composite.
ATG4D Antibody - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~52.9kDa ATG4D protein using Rabbit Anti-ATG4D Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG4D Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~52.9kDa.
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Polyclonal Rabbit anti‑Human ATG4D Antibody (FITC, IF, WB) LS‑C776182

Polyclonal Rabbit anti‑Human ATG4D Antibody (FITC, IF, WB) LS‑C776182

Antibody:
ATG4D Rabbit anti-Human Polyclonal (FITC) Antibody
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
FITC, Unmodified
Other formats:
Price
Catalog Number
$473
LS-C776182-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ATG4D Rabbit anti-Human Polyclonal (FITC) Antibody
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
FITC, Unmodified
Other formats:

Specifications

Description
ATG4D antibody LS-C776182 is an FITC-conjugated rabbit polyclonal antibody to ATG4D from human. It is reactive with human and mouse. Validated for IF and WB.
Target
Human ATG4D
Synonyms
ATG4D | APG4-D | APG4D | AUTL4 | APG4 autophagy 4 homolog D | Cysteine protease ATG4D | Autophagin-4
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
FITC. Also available conjugated with APC, Atto 390, Atto 488, Atto 594, Biotin, PerCP, RPE.
Purification
Peptide Affinity Purified
Modifications
Unmodified
Immunogen
Synthetic peptide from the C-Terminus of human ATG4D
Specificity
Detects ~52 kDa.
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Usage
Applications should be user optimized.
Presentation
PBS, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ATG4D
Q86TL0 NM_032885 NP_116274.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/5/2024