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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C156602-400 400 µl $325 
ATG4D Antibody - ATG4D Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.
ATG4D Antibody - Fluorescent image of U251 cells stained with ATG4D antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG4D primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). ATG4D immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
ATG4D Antibody - ATG4D Antibody western blot of mouse liver tissue lysates (35 ug/lane). The ATG4D antibody detected the ATG4D protein (arrow).
ATG4D Antibody - ATG4D Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.
ATG4D Antibody - Fluorescent image of U251 cells stained with ATG4D antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG4D primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). ATG4D immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
ATG4D Antibody - ATG4D Antibody western blot of mouse liver tissue lysates (35 ug/lane). The ATG4D antibody detected the ATG4D protein (arrow).
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ATG4D Antibody (aa42‑71) LS‑C156602

ATG4D Antibody (aa42‑71) LS‑C156602

Antibody:
Rabbit Polyclonal to Human ATG4D
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C156602-400
400 µl
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human ATG4D
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
ATG4D antibody LS-C156602 is an unconjugated rabbit polyclonal antibody to ATG4D from human and mouse. Validated for IF, IHC and WB.
Target
Human ATG4D
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:200)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E995 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C156602. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa42-71
Specificity
This ATG4D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-71 amino acids from the N-terminal region of human ATG4D.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ATG4D
Q86TL0 NM_032885 NP_116274.3

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Requested From: United States
Date Requested: 11/12/2019