ATF2 antibody LS-C3468 is an unconjugated rabbit polyclonal antibody to ATF2 from human, mouse and rat. Validated for ELISA, ICC, IHC, IP and WB.
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
- IHC - Paraffin
- ICC (1:5000)
- Western blot (1:1000)
- Immunoprecipitation (1:250)
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including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
ATF2 antibody was raised against synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr71 of human ATF2.
Detects endogenous levels of ATF-2 only when activated by phosphorylation at Thr71. Does not cross-react with phosphorylated c-Jun, CREB or other transcription factors. It recognizes both Thr69/Thr71 dually phosphorylated ATF-2 and Thr71 singly phosphorylated ATF-2 equally well. Species cross-reactivity: Human, mouse and rat.
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:250. Immunohistochemistry: 1:100 (Floating). Immunohistochemistry: 1:50 (Paraffin). Immunocytochemistry: 1:5000 (ABC detection method).
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.