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Catalog Number Size Price
LS-C209642-100 100 µg (1 mg/ml) $567 
Asparaginase Antibody - Western Blot of Rabbit anti-L-Asparaginase Antibody. Lane 1: L-Asparaginase. Lane 2: none. Load: 100 ng per lane. Primary antibody: L-Asparaginase antibody at 1:1000 for overnight at 4 degrees C. Secondary antibody: DyLight alpha 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 32 kDa for L-Asparaginase. Other band(s): L-Asparaginase splice variants and isoforms.

Polyclonal Rabbit anti‑E. coli Asparaginase Antibody (WB) LS‑C209642

Polyclonal Rabbit anti‑E. coli Asparaginase Antibody (WB) LS‑C209642

Antibody:
Asparaginase Rabbit anti-E. coli Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
E. coli
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C209642-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Asparaginase Rabbit anti-E. coli Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
E. coli
Format:
Unconjugated, Unmodified

Specifications

Description
Asparaginase antibody LS-C209642 is an unconjugated rabbit polyclonal antibody to e. coli Asparaginase. Validated for ELISA, IP and WB.
Target
E. coli Asparaginase
Host
Rabbit
Reactivity
E. coli (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Unmodified
Immunogen
Asparaginase [E. coli]
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Asparaginase [E. coli]. Cross reactivity against Asparaginase from other tissues and species may occur but have not been specifically determined.
Applications
  • Western blot (1:500 - 1:3000)
  • Immunoprecipitation (1:100)
  • ELISA (1:5000 - 1:25000)
Usage
Anti-L-Asparaginase antibody has been assayed against 1.0 ug of Asparaginase [E. coli] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:100000 to 1:400000 is suggested for this product.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/1/2024