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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ARHGDIB / D4 GDI Antibody (clone 10D774) LS‑C515
Note: This antibody replaces LS-C64482, LS-C14796
D4 GDI antibody LS-C515 is an unconjugated mouse monoclonal antibody to D4 GDI (ARHGDIB) from human, bat, bovine and other species. Validated for Flow and WB.
Catalog
Size
Price
LS-C515-100
100 µg (0.5 mg/ml)
$335

Popular ARHGDIB / D4 GDI Products

Western blot analysis: HL60 cells were treated for different time periods with 10 ng/ml of anti-Fas mAb (antibody, Clone DX2). Harvested cells were lysed and resolved by SDS-PAGE and transferred onto membrane. D4-GDI cleavage was detected by antibody.
Species: Human, Mouse
Applications: IHC, Western blot, Immunoprecipitation, Flow Cytometry
Antibody
Species: Human, Mouse
Applications: IHC, Western blot, Immunoprecipitation, Flow Cytometry
Antibody
Species: Human
Applications: ELISA
Antibody
Species:
Applications: Western blot, ELISA

Product Description

D4 GDI antibody LS-C515 is an unconjugated mouse monoclonal antibody to D4 GDI (ARHGDIB) from human, bat, bovine and other species. Validated for Flow and WB.

Specifications

Target
Human ARHGDIB / D4 GDI
Synonyms
ARHGDIB, D4 GDI, D4-GDI, GDIA2, Ly-GDI, RAP1GN1, Rho-GDI beta, RhoGDI2, Rho GDI 2, D4, GDID4, LYGDI
Host
Mouse
Reactivity
Human, Monkey, Bat, Bovine, Dog, Horse, Pig, Chicken (tested or 100% immunogen sequence identity)
Predicted
Rabbit (at least 90% immunogen sequence identity)
Clonality
IgG Monoclonal [10D774]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (1 - 2 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
Immunogen
A synthetic peptide corresponding to amino acids 125-137 (YVQHTYRTGVKVD) of human D4-GDI, GenBank no. ref|NP_001166.3|. The peptide sequence used for immunogen is 100% conserved in human, dog, cow, chick, and horse. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Ferret, Panda, Dog, Cat, Bovine, Horse, Pig, Turkey, Chicken, Lizard (100%); Rabbit, Opossum, Guinea pig (92%); Mouse, Elephant, Zebra finch (85%); Rat (77%).
Specificity
A 28 kD band is observed.
Presentation
PBS, 0.02% Sodium Azide, 0.02% Gelatin
Storage
Short term: 4°C; Long term: -70°C.
Restrictions
For research use only.
About ARHGDIB / D4 GDI
Members of the Rho (or ARH) protein family (see MIM 165390) and other Ras-related small GTP-binding proteins (see MIM 179520) are involved in diverse cellular events, including cell signaling, proliferation, cytoskeletal organization, and secretion. The GTP-binding proteins are active only in the GTP-bound state. P52566 NM_001175 NP_001166.3


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Images

Western blot

Western blot of antibody in whole Jurkat cell lysate.
Western blot of antibody in whole Jurkat cell lysate.

Flow Cytometry

HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the
HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the

Western blot

Western blot of antibody in whole Jurkat cell lysate.
Western blot of antibody in whole Jurkat cell lysate.

Flow Cytometry

HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the
HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the

Western blot

Western blot of antibody in whole Jurkat cell lysate.
Western blot of antibody in whole Jurkat cell lysate.

Flow Cytometry

HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the
HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the

Western blot

Western blot of antibody in whole Jurkat cell lysate.
Western blot of antibody in whole Jurkat cell lysate.

Flow Cytometry

HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the
HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the

Requested From: United States
Date Requested: 10/18/2018

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