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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ARHGAP35 / GRLF1 Antibody (phospho‑Tyr1105) LS‑C199023
GRLF1 antibody LS-C199023 is an unconjugated rabbit polyclonal antibody to GRLF1 (ARHGAP35) from human, mouse and rat. Validated for IHC, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C199023-50
50 µl (1 mg/ml)
$265
LS-C199023-100
100 µl (1 mg/ml)
$325

Popular ARHGAP35 / GRLF1 Products

Antibody
Species: Human, Mouse, Rat
Applications: Western blot
Western blot of Phospho-GRF-1 (Y1105) antibody
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA
Antibody
Species: Human, Mouse, Rat
Applications: IHC, ELISA
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GRF-1 Antibody. The picture on the right is blocked with the synthesized peptide.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

GRLF1 antibody LS-C199023 is an unconjugated rabbit polyclonal antibody to GRLF1 (ARHGAP35) from human, mouse and rat. Validated for IHC, Peptide-ELISA and WB.

Specifications

Target
Human ARHGAP35 / GRLF1
Synonyms
ARHGAP35, GRF-1, p190-A, p190A, p190ARhoGAP, Rho GAP p190A, p190RhoGAP, GRF1, GRLF1, KIAA1722
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide derived from human GRF-1 around the phosphorylation site of Tyr1105. (AA Range: 1071-1120)
Epitope
pTyr1105
Specificity
GRF-1 (Phospho-Tyr1105) Antibody detects endogenous levels of GRF-1 only when phosphorylated at tyrosine1105.
Usage
Western Blot: Predicted MW 172 kDa
Presentation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
About ARHGAP35 / GRLF1
The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. Q9NRY4 NM_004491 NP_004482.4


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Images

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GRF-1 (Phospho-Tyr1105) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GRF-1 (Phospho-Tyr1105) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot

Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using GRF-1 (Phospho-Tyr1105) Antibody. The lane on the right is blocked with the phospho peptide.
Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using GRF-1 (Phospho-Tyr1105) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GRF-1 (Phospho-Tyr1105) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GRF-1 (Phospho-Tyr1105) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot

Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using GRF-1 (Phospho-Tyr1105) Antibody. The lane on the right is blocked with the phospho peptide.
Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using GRF-1 (Phospho-Tyr1105) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GRF-1 (Phospho-Tyr1105) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GRF-1 (Phospho-Tyr1105) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot

Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using GRF-1 (Phospho-Tyr1105) Antibody. The lane on the right is blocked with the phospho peptide.
Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using GRF-1 (Phospho-Tyr1105) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GRF-1 (Phospho-Tyr1105) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GRF-1 (Phospho-Tyr1105) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot

Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using GRF-1 (Phospho-Tyr1105) Antibody. The lane on the right is blocked with the phospho peptide.
Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using GRF-1 (Phospho-Tyr1105) Antibody. The lane on the right is blocked with the phospho peptide.

Requested From: United States
Date Requested: 11/12/2018

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