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Catalog Number Size Price
LS-C773886-100 100 µg (1 mg/ml) $473 
APG4B / ATG4B Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4B Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4B Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4B Antibody (D) Composite.
APG4B / ATG4B Antibody - Western blot analysis of Mouse Brain cell lysates showing detection of 44.3 kDa ATG4B protein using Rabbit Anti-ATG4B Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4B Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 44.3 kDa.
APG4B / ATG4B Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4B Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4B Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4B Antibody (D) Composite.
APG4B / ATG4B Antibody - Western blot analysis of Mouse Brain cell lysates showing detection of 44.3 kDa ATG4B protein using Rabbit Anti-ATG4B Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4B Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 44.3 kDa.
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Polyclonal Rabbit anti‑Human APG4B / ATG4B Antibody (FITC, IF, WB) LS‑C773886

Polyclonal Rabbit anti‑Human APG4B / ATG4B Antibody (FITC, IF, WB) LS‑C773886

Antibody:
APG4B / ATG4B Rabbit anti-Human Polyclonal (FITC) Antibody
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
FITC, Unmodified
Other formats:
Price
Catalog Number
$473
LS-C773886-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
APG4B / ATG4B Rabbit anti-Human Polyclonal (FITC) Antibody
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
FITC, Unmodified
Other formats:

Specifications

Description
ATG4B antibody LS-C773886 is an FITC-conjugated rabbit polyclonal antibody to ATG4B (APG4B) from human. It is reactive with human and mouse. Validated for IF and WB.
Target
Human APG4B / ATG4B
Synonyms
ATG4B | APG4 autophagy 4 homolog B | AUTL1 | Autophagin-1 | Cysteine protease ATG4B | HAPG4B | APG4B | KIAA0943
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
FITC. Also available conjugated with AP, APC, Atto 390, Atto 488, Atto 594, Biotin, PerCP, RPE.
Purification
Peptide Affinity Purified
Modifications
Unmodified
Immunogen
Synthetic peptide from the N-Terminus of Human ATG4B (aa. 62-73)
Specificity
Detects ~50 kDa.
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Usage
Applications should be user optimized.
Presentation
PBS, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APG4B / ATG4B
Q9Y4P1 NM_013325 NP_037457.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/23/2024