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Catalog Number Size Price
LS-C498425-50 50 µg $294 
LS-C498425-100 100 µg $360 
APG4B / ATG4B Antibody - Immunohistochemistry of paraffin-embedded human colon cancer using ATG4B Antibody at dilution of 1:100
APG4B / ATG4B Antibody - Immunohistochemistry of paraffin-embedded human pancreatic cancer using ATG4B Antibody at dilution of 1:100
APG4B / ATG4B Antibody - Immunofluorescent analysis of A549 cells using ATG4B Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
APG4B / ATG4B Antibody - Positive WB detected in:Mouse brain tissue;All lanes:ATG4B antibody at 3.4?g/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 45,53,32,38,43 KDa;Observed band size: 45 KDa;
APG4B / ATG4B Antibody - Immunohistochemistry of paraffin-embedded human colon cancer using ATG4B Antibody at dilution of 1:100
APG4B / ATG4B Antibody - Immunohistochemistry of paraffin-embedded human pancreatic cancer using ATG4B Antibody at dilution of 1:100
APG4B / ATG4B Antibody - Immunofluorescent analysis of A549 cells using ATG4B Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
APG4B / ATG4B Antibody - Positive WB detected in:Mouse brain tissue;All lanes:ATG4B antibody at 3.4?g/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 45,53,32,38,43 KDa;Observed band size: 45 KDa;
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Polyclonal Rabbit anti‑Human APG4B / ATG4B Antibody (IHC, IF, WB) LS‑C498425

Polyclonal Rabbit anti‑Human APG4B / ATG4B Antibody (IHC, IF, WB) LS‑C498425

Antibody:
APG4B / ATG4B Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$294
LS-C498425-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
APG4B / ATG4B Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
ATG4B antibody LS-C498425 is an unconjugated rabbit polyclonal antibody to ATG4B (APG4B) from human. It is reactive with human and mouse. Validated for ELISA, IF, IHC and WB.
Target
Human APG4B / ATG4B
Synonyms
ATG4B | APG4 autophagy 4 homolog B | AUTL1 | Autophagin-1 | Cysteine protease ATG4B | HAPG4B | APG4B | KIAA0943
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant Human Cysteine protease ATG4B protein (350-400AA)
Specificity
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Applications
  • IHC
  • Immunofluorescence
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Storage
Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APG4B / ATG4B
Q9Y4P1 NM_013325 NP_037457.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/29/2024