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Antibodies > APG4B / ATG4B > LS-C156592
 
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Catalog Number Size Price
LS-C156592-400 400 µl $393 
APG4B / ATG4B Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
APG4B / ATG4B Antibody - Fluorescent image of U251 cells stained with ATG4B antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG4B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG4B immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
APG4B / ATG4B Antibody - Western blot of anti-APG4B antibody in HeLa cell lysate. APG4B (arrow) was detected using the purified antibody.
APG4B / ATG4B Antibody - Western blot of anti-hAPG4B-373 antibody in 293 cell line lysates transiently transfected with the ATG4B gene (2 ug/lane). hAPG4B-373(arrow) was detected using the purified antibody.
APG4B / ATG4B Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
APG4B / ATG4B Antibody - Fluorescent image of U251 cells stained with ATG4B antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG4B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG4B immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
APG4B / ATG4B Antibody - Western blot of anti-APG4B antibody in HeLa cell lysate. APG4B (arrow) was detected using the purified antibody.
APG4B / ATG4B Antibody - Western blot of anti-hAPG4B-373 antibody in 293 cell line lysates transiently transfected with the ATG4B gene (2 ug/lane). hAPG4B-373(arrow) was detected using the purified antibody.
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Polyclonal Rabbit anti‑Human APG4B / ATG4B Antibody (aa358‑390, IHC, IF, WB) LS‑C156592

Polyclonal Rabbit anti‑Human APG4B / ATG4B Antibody (aa358‑390, IHC, IF, WB) LS‑C156592

Submit Review LSBio Guarantee
Antibody:
APG4B / ATG4B Rabbit anti-Human Polyclonal (aa358-390) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C156592-400
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
APG4B / ATG4B Rabbit anti-Human Polyclonal (aa358-390) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ATG4B antibody LS-C156592 is an unconjugated rabbit polyclonal antibody to human ATG4B (APG4B) (aa358-390). Validated for IF, IHC and WB.
Target
Human APG4B / ATG4B
Synonyms
ATG4B | APG4 autophagy 4 homolog B | AUTL1 | Autophagin-1 | Cysteine protease ATG4B | HAPG4B | APG4B | KIAA0943
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa358-390
Specificity
This ATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 358-390 amino acids from the C-terminal region of human ATG4B.
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APG4B / ATG4B
Q9Y4P1 NM_013325 NP_037457.3

Publications (0)

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APG4B / ATG4B Antibody - A: Marker, B: Rap Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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See all APG4B / ATG4B Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/29/2024
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