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Catalog Number Size Price
LS-C815739-100 100 µl $412 
APEX1 / APE1 Antibody - Immunofluorescence staining of Apex1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-MOUSE Apex1 polyclonal antibody (dilution ratio: 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.
APEX1 / APE1 Antibody - Apex1 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. 4 uL anti-Apex1 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-Apex1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 39 kDa.
APEX1 / APE1 Antibody - Immunofluorescence staining of Apex1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-MOUSE Apex1 polyclonal antibody (dilution ratio: 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.
APEX1 / APE1 Antibody - Apex1 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. 4 uL anti-Apex1 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-Apex1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 39 kDa.
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Polyclonal Rabbit anti‑Mouse APEX1 / APE1 Antibody (Preservative Free, IHC, IF, WB) LS‑C815739

Polyclonal Rabbit anti‑Mouse APEX1 / APE1 Antibody (Preservative Free, IHC, IF, WB) LS‑C815739

Antibody:
APEX1 / APE1 Rabbit anti-Mouse Polyclonal (Preservative Free) Antibody
Application:
IHC-P, ICC, IF, WB, IP
Reactivity:
Mouse
Format:
Unconjugated, Preservative Free
Price
Catalog Number
$412
LS-C815739-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
APEX1 / APE1 Rabbit anti-Mouse Polyclonal (Preservative Free) Antibody
Application:
IHC-P, ICC, IF, WB, IP
Reactivity:
Mouse
Format:
Unconjugated, Preservative Free

Specifications

Description
APE1 antibody LS-C815739 is an unconjugated rabbit polyclonal antibody to mouse APE1 (APEX1). Validated for ICC, IF, IHC, IP and WB.
Target
Mouse APEX1 / APE1
Synonyms
APEX1 | AP endonuclease class I | AP lyase | APE | APE-1 | APEN | APE1 | APEX nuclease | Redox factor-1 | AP endonuclease 1 | APEX | APX | Protein REF-1 | REF-1 | REF1
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Preservative Free
Immunogen
A synthetic peptide corresponding to the N-Terminus of Mouse APEX1/APE1/Ref-1
Specificity
Mouse APE1/APEX1
Applications
  • IHC - Paraffin (1:200 - 1:1000)
  • ICC (1:300 - 1:10000)
  • Immunofluorescence (1:300 - 1:10000)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.2 µm filtered solution in PBS
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APEX1 / APE1
P27695 NM_001641 NP_001632.2

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Requested From: United States
Date Requested: 5/1/2024