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Antibodies > APEX1 / APE1 > LS-C746818
 
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Catalog Number Size Price
LS-C746818-20 20 µl $287 
LS-C746818-100 100 µl $431 
APEX1 / APE1 Antibody - Immunofluorescence analysis of A549 cells using APEX1 antibody.
APEX1 / APE1 Antibody - Immunofluorescence analysis of HeLa cells using APEX1 antibody. Blue: DAPI for nuclear staining.
APEX1 / APE1 Antibody - Western blot analysis of extracts from normal (control) and APEX1 knockout (KO) 293T cells, using APEX1 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
APEX1 / APE1 Antibody - Western blot analysis of extracts of various cell lines, using APEX1 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
APEX1 / APE1 Antibody - Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug APEX1 antibody. Western blot was performed from the immunoprecipitate using APEX1 antibody at a dilition of 1:1000.
APEX1 / APE1 Antibody - Immunofluorescence analysis of A549 cells using APEX1 antibody.
APEX1 / APE1 Antibody - Immunofluorescence analysis of HeLa cells using APEX1 antibody. Blue: DAPI for nuclear staining.
APEX1 / APE1 Antibody - Western blot analysis of extracts from normal (control) and APEX1 knockout (KO) 293T cells, using APEX1 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
APEX1 / APE1 Antibody - Western blot analysis of extracts of various cell lines, using APEX1 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
APEX1 / APE1 Antibody - Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug APEX1 antibody. Western blot was performed from the immunoprecipitate using APEX1 antibody at a dilition of 1:1000.
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Polyclonal Rabbit anti‑Human APEX1 / APE1 Antibody (IF, WB) LS‑C746818

Polyclonal Rabbit anti‑Human APEX1 / APE1 Antibody (IF, WB) LS‑C746818

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Antibody:
APEX1 / APE1 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$287
LS-C746818-20
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
APEX1 / APE1 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
APE1 antibody LS-C746818 is an unconjugated rabbit polyclonal antibody to human APE1 (APEX1). Validated for IF, IP and WB.
Target
Human APEX1 / APE1
Synonyms
APEX1 | AP endonuclease class I | AP lyase | APE | APE-1 | APEN | APE1 | APEX nuclease | Redox factor-1 | AP endonuclease 1 | APEX | APX | Protein REF-1 | REF-1 | REF1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-318 of human APEX1 (NP_542380.1). MPKRGKKGAVAEDGDELRTEPEAKKSKTAAKKNDKEAAGEGPALYEDPPDQKTSPSGKPATLKICSWNVDGLRAWIKKKGLDWVKEEAPDILCLQETKCSENKLPAELQELPGLSHQYWSAPSDKEGYSGVGLLSRQCPLKVSYGIGDEEHDQEGRVIVAEFDSFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKGLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFGELLQAVPLADSFRHLYPNTPYAYTFWTYMMNARSKNVGWRLDYFLLSHSLLPALCDSKIRSKALGSDHCPITLYLAL
Applications
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:50 - 1:200)
Usage
The predicted MW is 35kDa, while the observed MW by Western blot was 39kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APEX1 / APE1
P27695 NM_001641 NP_001632.2

Publications (0)

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Species: Human, Mouse, Rat
Applications: IHC, IHC - Frozen, ICC, Western blot, Immunoprecipitation
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IHC‑plus™ APEX1 / APE1 Antibody (aa2‑15) LS‑B3924
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APEX1 / APE1 Antibody LS‑C136888
Species: Human
Applications: Western blot, ELISA
APEX1 / APE1 Antibody - APEX1 / APE1 antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
APEX1 / APE1 Antibody (aa215‑264) LS‑C116767
Species: Human
Applications: Western blot
APEX1 / APE1 Antibody - APEX1 / APE1 antibody ARP32651_T100-NP_542379-APEX1 (APEX nuclease (multifunctional DNA repair enzyme) 1) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human lung. This image was taken for the unconjugated form of this product. Other forms have not been tested.
APEX1 / APE1 Antibody (aa79‑128) LS‑C30761
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Applications: IHC, IHC - Paraffin, Western blot
Product
APEX1 / APE1 Antibody (aa219‑318) LS‑C306289
Species: Human
Applications: ELISA
See all APEX1 / APE1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/1/2024
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