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Catalog Number Size Price
LS-C392589-200 200 µl $393 
APEX1 / APE1 Antibody - Antibody staining APEX1 in human stomach sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
APEX1 / APE1 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with antibody at 1:25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1:200 dilution (green). Immunofluorescence image showing nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1:100 dilution (red).
APEX1 / APE1 Antibody - All lanes: Anti-APEX1 Antibody at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: HeLa whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: K562 whole cell lysate. Lane 5: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
APEX1 / APE1 Antibody - Antibody staining APEX1 in human stomach sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
APEX1 / APE1 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with antibody at 1:25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1:200 dilution (green). Immunofluorescence image showing nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1:100 dilution (red).
APEX1 / APE1 Antibody - All lanes: Anti-APEX1 Antibody at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: HeLa whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: K562 whole cell lysate. Lane 5: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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Monoclonal Mouse anti‑Human APEX1 / APE1 Antibody (clone 1518CT337.123.86.269.232, IHC, IF, WB) LS‑C392589

Monoclonal Mouse anti‑Human APEX1 / APE1 Antibody (clone 1518CT337.123.86.269.232, IHC, IF, WB) LS‑C392589

Antibody:
APEX1 / APE1 Mouse anti-Human Monoclonal (1518CT337.123.86.269.232) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C392589-200
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
APEX1 / APE1 Mouse anti-Human Monoclonal (1518CT337.123.86.269.232) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
APE1 antibody LS-C392589 is an unconjugated mouse monoclonal antibody to human APE1 (APEX1). Validated for IF, IHC, Peptide-ELISA and WB. Cited in 1 publication.
Target
Human APEX1 / APE1
Synonyms
APEX1 | AP endonuclease class I | AP lyase | APE | APE-1 | APEN | APE1 | APEX nuclease | Redox factor-1 | AP endonuclease 1 | APEX | APX | Protein REF-1 | REF-1 | REF1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
1518CT337.123.86.269.232
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:25)
  • Immunofluorescence (1:25)
  • Western blot (1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APEX1 / APE1
P27695 NM_001641 NP_001632.2

LSBio Ratings

APEX1 / APE1 Antibody (clone 1518CT337.123.86.269.232) for IHC, IF/Immunofluorescence, WB/Western LS-C392589 has an LSBio Rating of
Publications (4)

Publications (1)

APEX1 Expression as a Potential Diagnostic Biomarker of Clear Cell Renal Cell Carcinoma and Hepatobiliary Carcinomas. Kim JM, Yeo MK, Lim JS, Song IS, Chun K, Kim KH. Journal of Clinical Medicine. 2019 August;8:E1151.

Customer Reviews (0)


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Requested From: United States
Date Requested: 4/25/2024