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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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APBB1 / FE65 Antibody (Biotin) LS‑C141565
FE65 antibody LS-C141565 is a biotin-conjugated rabbit polyclonal antibody to FE65 (APBB1) from human, mouse and rat. Validated for IP and WB.
Catalog
Size
Price
LS-C141565-100
100 µl
$365
Description
FE65 antibody LS-C141565 is a biotin-conjugated rabbit polyclonal antibody to FE65 (APBB1) from human, mouse and rat. Validated for IP and WB.
Target
Rat APBB1 / FE65
Host
Rabbit
Reactivity
Rat, Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin. Also available Unconjugated or conjugated with DY650, DY488, DY550.
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • Immunoprecipitation (1:1000)
  • (applications tested for the base form of this product only)
Immunogen
APBB1 / FE65 antibody was raised against recombinant rat protein expressed in bacteria
Specificity
Fe65.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.05% Sodium Azide
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
About APBB1 / FE65
O00213 NM_001164 NP_001155.1

Popular APBB1 / FE65 Products

Immunohistochemistry of paraffin-embedded rat lung using APBB1 antibody at dilution of 1:100 (200x lens).
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
FE65 antibody IHC-paraffin. IHC(P): Rat Brain Tissue.
Species: Human, Mouse, Rat, Bovine, Guinea pig, Horse, Pig, Sheep
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry - Paraffin Image
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
Immunofluorescence analysis of Fe65 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Fe65 Rabbit Polyclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Species: Mouse
Applications: Immunofluorescence, Western blot

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Images

Western blot

Detection of protein in HEK 293 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of protein in HEK 293 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of protein in HEK 293 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of protein in HEK 293 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of protein in HEK 293 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of protein in HEK 293 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of protein in HEK 293 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of protein in HEK 293 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/19/2018
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