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Antibodies > ANXA6/Annexin A6/Annexin VI > LS-C662953
 
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Catalog Number Size Price
LS-C662953-10 10 µg $318 
LS-C662953-100 100 µg $470 
ANXA6/Annexin A6/Annexin VI Antibody - IHC analysis of Annexin VI using anti-Annexin VI antibody. Annexin VI was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-Annexin VI Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ANXA6/Annexin A6/Annexin VI Antibody - IHC analysis of Annexin VI using anti-Annexin VI antibody
ANXA6/Annexin A6/Annexin VI Antibody - IHC analysis of Annexin VI using anti-Annexin VI antibody
ANXA6/Annexin A6/Annexin VI Antibody - Western blot analysis of Annexin VI using anti-Annexin VI antibody
ANXA6/Annexin A6/Annexin VI Antibody - IF analysis of Annexin VI using anti-Annexin VI antibody Annexin VI was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-Annexin VI Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
ANXA6/Annexin A6/Annexin VI Antibody - IF analysis of Annexin VI using anti-Annexin VI antibody Annexin VI was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-Annexin VI Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
ANXA6/Annexin A6/Annexin VI Antibody - IF analysis of Annexin VI using anti-Annexin VI antibody Annexin VI was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-Annexin VI Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
ANXA6/Annexin A6/Annexin VI Antibody - IF analysis of Annexin VI using anti-Annexin VI antibody Annexin VI was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-Annexin VI Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
ANXA6/Annexin A6/Annexin VI Antibody - IF analysis of Annexin VI using anti-Annexin VI antibody Annexin VI was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-Annexin VI Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
ANXA6/Annexin A6/Annexin VI Antibody - IHC analysis of Annexin VI using anti-Annexin VI antibody
ANXA6/Annexin A6/Annexin VI Antibody - Western blot analysis of Annexin VI using anti-Annexin VI antibody
ANXA6/Annexin A6/Annexin VI Antibody - Flow Cytometry analysis of HepG2 cells using anti-Annexin VI antibody. Overlay histogram showing HepG2 cells stained with anti-Annexin VI antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Annexin VI Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
ANXA6/Annexin A6/Annexin VI Antibody - IHC analysis of Annexin VI using anti-Annexin VI antibody. Annexin VI was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-Annexin VI Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ANXA6/Annexin A6/Annexin VI Antibody - IHC analysis of Annexin VI using anti-Annexin VI antibody
ANXA6/Annexin A6/Annexin VI Antibody - IHC analysis of Annexin VI using anti-Annexin VI antibody
ANXA6/Annexin A6/Annexin VI Antibody - Western blot analysis of Annexin VI using anti-Annexin VI antibody
ANXA6/Annexin A6/Annexin VI Antibody - IF analysis of Annexin VI using anti-Annexin VI antibody Annexin VI was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-Annexin VI Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
ANXA6/Annexin A6/Annexin VI Antibody - IF analysis of Annexin VI using anti-Annexin VI antibody Annexin VI was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-Annexin VI Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
ANXA6/Annexin A6/Annexin VI Antibody - IF analysis of Annexin VI using anti-Annexin VI antibody Annexin VI was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-Annexin VI Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
ANXA6/Annexin A6/Annexin VI Antibody - IF analysis of Annexin VI using anti-Annexin VI antibody Annexin VI was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-Annexin VI Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
ANXA6/Annexin A6/Annexin VI Antibody - IF analysis of Annexin VI using anti-Annexin VI antibody Annexin VI was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-Annexin VI Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
ANXA6/Annexin A6/Annexin VI Antibody - IHC analysis of Annexin VI using anti-Annexin VI antibody
ANXA6/Annexin A6/Annexin VI Antibody - Western blot analysis of Annexin VI using anti-Annexin VI antibody
ANXA6/Annexin A6/Annexin VI Antibody - Flow Cytometry analysis of HepG2 cells using anti-Annexin VI antibody. Overlay histogram showing HepG2 cells stained with anti-Annexin VI antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Annexin VI Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human ANXA6/Annexin A6/Annexin VI Antibody (IHC, WB) LS‑C662953

Polyclonal Rabbit anti‑Human ANXA6/Annexin A6/Annexin VI Antibody (IHC, WB) LS‑C662953

Submit Review LSBio Guarantee
Antibody:
ANXA6/Annexin A6/Annexin VI Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662953-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ANXA6/Annexin A6/Annexin VI Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Annexin VI antibody LS-C662953 is an unconjugated rabbit polyclonal antibody to human Annexin VI (ANXA6 / Annexin A6). Validated for ELISA, IHC and WB.
Target
Human ANXA6/Annexin A6/Annexin VI
Synonyms
ANXA6 | 67 kDa calelectrin | Annexin VI (p68) | Annexin A6 | Annexin VI | ANX6 | Calcium-binding protein p68 | Calelectrin | CBP68 | Chromobindin-20 | Calphobindin-II | Lipocortin VI | p70 | Protein III | Annexin-6 | Calphobindin II | CPB-II | p68
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
E. coli-derived human Annexin VI recombinant protein (Position: N395-L665).
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ANXA6/Annexin A6/Annexin VI
P08133 NM_001155 NP_001146.2

Publications (0)

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ANXA6/Annexin A6/Annexin VI Antibody - Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
ANXA6/Annexin A6/Annexin VI Antibody (Biotin) LS‑C316973
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ANXA6/Annexin A6/Annexin VI Antibody - Anti-Annexin VI antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
IHC‑plus™ ANXA6/Annexin A6/Annexin VI Antibody (aa1‑50) LS‑B7015
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
ANXA6/Annexin A6/Annexin VI Antibody - ANXA6/Annexin A6/Annexin VI antibody. IHC(F): Rat Spleen Tissue.
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Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot
ANXA6/Annexin A6/Annexin VI Antibody - Western Blot; Sample: Lane1: Human Serum; Lane2: Human Hela Cells.
ANXA6/Annexin A6/Annexin VI Antibody (aa2‑251, Biotin) LS‑C687002
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See all ANXA6/Annexin A6/Annexin VI Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/29/2024
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