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Antibodies > ANXA4 / Annexin IV > LS-C662221
 
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Catalog Number Size Price
LS-C662221-10 10 µg $318 
LS-C662221-100 100 µg $470 
ANXA4 / Annexin IV Antibody - IHC analysis of Annexin IV using anti-Annexin IV antibody. Annexin IV was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Annexin IV Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ANXA4 / Annexin IV Antibody - IHC analysis of Annexin IV using anti-Annexin IV antibody. Annexin IV was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Annexin IV Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ANXA4 / Annexin IV Antibody - IHC analysis of Annexin IV using anti-Annexin IV antibody. Annexin IV was detected in paraffin-embedded section of rat pancreas tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Annexin IV Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ANXA4 / Annexin IV Antibody - IHC analysis of Annexin IV using anti-Annexin IV antibody. Annexin IV was detected in paraffin-embedded section of mouse thymus tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Annexin IV Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ANXA4 / Annexin IV Antibody - IHC analysis of Annexin IV using anti-Annexin IV antibody. Annexin IV was detected in paraffin-embedded section of rat thymus tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Annexin IV Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ANXA4 / Annexin IV Antibody - Western blot analysis of Annexin IV using anti-Annexin IV antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin IV antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Annexin IV at approximately 36KD. The expected band size for Annexin IV is at 36KD.
ANXA4 / Annexin IV Antibody - Western blot - Anti-Annexin IV Picoband Antibody
ANXA4 / Annexin IV Antibody - IHC analysis of Annexin IV using anti-Annexin IV antibody. Annexin IV was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Annexin IV Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ANXA4 / Annexin IV Antibody - IHC analysis of Annexin IV using anti-Annexin IV antibody. Annexin IV was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Annexin IV Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ANXA4 / Annexin IV Antibody - IHC analysis of Annexin IV using anti-Annexin IV antibody. Annexin IV was detected in paraffin-embedded section of rat pancreas tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Annexin IV Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ANXA4 / Annexin IV Antibody - IHC analysis of Annexin IV using anti-Annexin IV antibody. Annexin IV was detected in paraffin-embedded section of mouse thymus tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Annexin IV Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ANXA4 / Annexin IV Antibody - IHC analysis of Annexin IV using anti-Annexin IV antibody. Annexin IV was detected in paraffin-embedded section of rat thymus tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Annexin IV Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ANXA4 / Annexin IV Antibody - Western blot analysis of Annexin IV using anti-Annexin IV antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin IV antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Annexin IV at approximately 36KD. The expected band size for Annexin IV is at 36KD.
ANXA4 / Annexin IV Antibody - Western blot - Anti-Annexin IV Picoband Antibody
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Polyclonal Rabbit anti‑Human ANXA4 / Annexin IV Antibody (WB) LS‑C662221

Polyclonal Rabbit anti‑Human ANXA4 / Annexin IV Antibody (WB) LS‑C662221

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Antibody:
ANXA4 / Annexin IV Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662221-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ANXA4 / Annexin IV Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Annexin IV antibody LS-C662221 is an unconjugated rabbit polyclonal antibody to human Annexin IV (ANXA4). Validated for WB.
Target
Human ANXA4 / Annexin IV
Synonyms
ANXA4 | 35-beta calcimedin | ANX4 | Annexin A4 | Annexin IV | Annexin-4 | Chromobindin-4 | Endonexin I | Lipocortin IV | p32.5 | PIG28 | Proliferation-inducing gene 28 | Protein II | ZAP36 | p33/41 | PAP-II | PP4-X
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Annexin IV (119-152 aa EEIRRISQTYQQQYGRSLEDDIRSDTSFMFQRVL), different from the related mouse and rat sequences by three amino acids.
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ANXA4 / Annexin IV
P09525 NM_001153 NP_001144.1

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Featured Products

ANXA4 / Annexin IV Antibody - Anti-Annexin IV antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ ANXA4 / Annexin IV Antibody (clone 1D3) LS‑B4279
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
ANXA4 / Annexin IV Antibody - Immunohistochemistry of paraffin-embedded Human gastric cancer using ANXA4 Polyclonal Antibody at dilution of 1:25.
ANXA4 / Annexin IV Antibody LS‑C403819
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA
ANXA4 / Annexin IV Antibody - Anti-ANXA4 / Annexin IV antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
ANXA4 / Annexin IV Antibody (aa108‑157, FITC) LS‑C434406
Species: Human, Chimpanzee, Gibbon, Mouse, Rat, Bat, Bovine, Dog, Pig
Applications: IHC, IHC - Paraffin, Western blot
ANXA4 / Annexin IV ELISA Kit
Human ANXA4 / Annexin IV (Sandwich ELISA) ELISA Kit - LS-F6848
Reactivity: Human
Range: 18.75-1200 pg/ml
ANXA4 / Annexin IV ELISA Kit
Human ANXA4 / Annexin IV (Sandwich ELISA) ELISA Kit - LS-F34445
Reactivity: Human
Range: 78.125-5000 pg/ml
ANXA4 / Annexin IV Protein - Recombinant Annexin A4 By SDS-PAGE
Mouse ANXA4 / Annexin IV Protein (Recombinant His) (aa151-313) - LS-G15547
Source: E. coli
Tag: His
See all ANXA4 / Annexin IV Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/29/2024
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