Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Monkey, Bovine, Pig (tested or 100% immunogen sequence identity)
Mouse, Rat, Dog (at least 90% immunogen sequence identity)
Specificity and Use
ZNF697 antibody was raised against synthetic peptide C-RHLSLGSRHRGDK from an internal region of human ZNF697 (NP_001073939.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Ferret, Elephant, Bovine, Pig (100%); Chimpanzee, Mouse, Rat, Panda, Dog, Cat (92%).
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Preliminary experiments gave bands at approx 75kD and 50kD in HeLa nuclear lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 60.5kD according to NP_001073939.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).