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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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ZNF385B / ZNF533 Antibody (aa254‑303) LS‑C82632
ZNF533 antibody LS-C82632 is an unconjugated rabbit polyclonal antibody to ZNF533 (ZNF385B) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C82632-50
50 µg
Unavailable

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Product Description

ZNF533 antibody LS-C82632 is an unconjugated rabbit polyclonal antibody to ZNF533 (ZNF385B) from human, mouse, rat and other species. Validated for WB.
About ZNF385B / ZNF533
May play a role in p53/TP53-mediated apoptosis. Q569K4 AA460775 Q569K4

ZNF385B Antibody, Zinc finger protein 385B Antibody, Zinc finger protein 533 Antibody, ZNF533 Antibody, ZFN533 Antibody


Specifications

Target
Human ZNF385B / ZNF533
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Chicken, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa254-303 of human ZNF385B (Q569K4, NP_001106869). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%); Opossum, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Stickleback, Zebrafish (92%); Panda, Lizard (85%).
Blocking Peptide
LS-E18343 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C82632. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa254-303
Specificity
Human ZNF385B
Usage
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Fetal liver lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal liver lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal liver lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal liver lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal liver lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal liver lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal liver lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal liver lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/18/2018

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