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Anti-ZNF207 Antibody (aa360-409) LS-C101451

Catalog Size Price
LS-C101451-50 50 µg Unavailable

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20 ZNF207 Antibodies

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Immunoperoxidase of monoclonal antibody to ZNF207 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 3 ug/ml]
Anti-ZNF207 Antibody (aa88-116) LS-C160836
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Fluorescent confocal image of A549 cell stained with ZNF207 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZNF207 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ZNF207 immunoreactivity is localized to Nucleus significantly.
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Anti-ZNF207 Antibody LS-C680081
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Immunofluorescent analysis of Hela cells diluted at 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

100% Guaranteed
Rabbit Polyclonal (IgG) to Human ZNF207
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human ZNF207
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Xenopus (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

ZNF207 antibody was raised against synthetic peptide located between aa360-409 of human ZNF207 (O43670, NP_003448). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Zebrafish (100%); Xenopus, Stickleback (92%).
LS-E11858 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C101451. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa360-409
Human ZNF207
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About ZNF207

O43670 NM_003457 NP_003448.1

ZNF207 Antibody, D38 Antibody, Zinc finger protein 207 Antibody

Kinetochore-binding protein involved in spindle-assembly checkpoint signaling by regulating mitotic chromosome alignment and mediating the stability and kinetochore loading of BUB3. Mechanisms by which BUB3 is protected are unclear: according to a first report, ZNF207/BuGZ may act by blocking ubiquitination and proteasomal degradation of BUB3 (PubMed:24462186). According to another report, the stabilization is independent of the proteasome (PubMed:24462187).

Western blot

ZNF207 antibody LS-C101451 Western blot of Fetal Muscle lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ZNF207 antibody LS-C101451 Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 4/21/2018

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