Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 3D5] (IgG2a,k) to Human ZIC4
Immunofluorescence, Western blot, ELISA
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [3D5]
Purified from ascites by Protein A
Immunofluorescence (7 µg/ml)
IHC - Paraffin
Specificity and Use
ZIC4 antibody was raised against zIC4 (NP_115529, 249 a.a. ~ 320 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Western Blot (Cell lysate) - positive control Hela NE. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections, 3 ug/ml) - positive control human placenta. Immunofluorescence (7 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).
PBS, pH 7.4
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
ZIC4 Antibody, Zic family member 4 Antibody, Zinc finger protein ZIC 4 Antibody
ZIC4 is a member of the ZIC family of C2H2-type zinc finger proteins. Members of this family are important during development, and have been associated with X-linked visceral heterotaxy and holoprosencephaly type 5. This gene is closely linked to the gene encoding zinc finger protein of the cerebellum 1, a related family member on chromosome 3. Heterozygous deletion of these linked genes is involved in Dandy-Walker malformation, which is a congenital cerebellar malformation.