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Anti-ZBTB33 / Kaiso Antibody (N-Terminus) IHC-plus™ LS-B1116

Note: This antibody replaces LS-C30237

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ZBTB33 / Kaiso Antibodies

Anti-ZBTB33 / Kaiso Antibody (N-Terminus) IHC-plus™ LS-B1116
Rabbit Polyclonal to Human ZBTB33 / Kaiso
Human, Monkey, Bovine, Dog, Guinea pig, Hamster, Horse, Pig
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-ZBTB33 / Kaiso Antibody LS-C71180
Mouse Monoclonal (IgG1) to Human ZBTB33 / Kaiso
Human, Mouse, Rat
Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Anti-ZBTB33 / Kaiso Antibody LS-C130320
Goat Polyclonal (IgG) to Human ZBTB33 / Kaiso
Human
Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human ZBTB33 / Kaiso
Human, Monkey, Bovine, Dog, Guinea pig, Hamster, Horse, Pig
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human ZBTB33 / Kaiso
Rabbit
Human, Monkey, Bovine, Dog, Guinea pig, Hamster, Horse, Pig (tested or 100% immunogen sequence identity)
Mouse, Rat, Rabbit (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

ZBTB33 / Kaiso antibody was raised against synthetic peptide from N-Terminus of human ZBTB33 (Q86T24, NP_006768). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Hamster, Panda, Dog, Bovine, Horse, Pig, Guinea pig (100%); Mouse, Rat, Rabbit, Opossum (92%); Chicken, Platypus (85%).
LS-E13998 - Lyophilized - 100 µg - $145.00
N-Terminus
Human ZBTB33 / Kaiso
Immunohistochemistry: LS-B1116 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1116 was determined to be 2.5 ug/ml.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl After adding water, will consist of PBS buffer with 2% sucrose
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About ZBTB33 / Kaiso

Q86T24 NM_006777 NP_006768.1

ZBTB33 Antibody, Kaiso Antibody, WUGSC:H_DJ525N14.1 Antibody, ZNF-kaiso Antibody, Kaiso transcription factor Antibody, ZNF348 Antibody

ZBTB33 / Kaiso is a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters.

Immunohistochemistry

Immunohistochemistry
Anti-ZBTB33 / Kaiso antibody IHC of human small intestine, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1116 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
ZBTB33 / Kaiso antibody LS-B1116 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
A: Marker, B: HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/7/2016

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