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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ZBTB33 / Kaiso Antibody (aa103‑584) LS‑C16122
Kaiso antibody LS-C16122 is an unconjugated mouse monoclonal antibody to Kaiso (ZBTB33) from human, mouse, rat and other species. Validated for IHC, IP and WB.
Antibody
Catalog
Size
Price
LS-C16122-200
200 µg
$620

Popular ZBTB33 / Kaiso Products

Anti-ZBTB33 / Kaiso antibody IHC of human small intestine, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1116 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Bovine, Dog, Guinea pig, Hamster, Horse, Pig
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Mouse, Human, Rat, Dog, Hamster, Chicken
Applications: IHC, Western blot, Immunoprecipitation
Antibody
Species: Mouse, Human, Rat, Dog, Chicken
Applications: Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Antibody
Species: Human, Mouse, Rat, Dog, Chicken
Applications: Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Antibody
Species: Human, Mouse, Rat, Dog, Chicken
Applications: Immunofluorescence, Western blot, Immunoprecipitation, ELISA

Product Description

Kaiso antibody LS-C16122 is an unconjugated mouse monoclonal antibody to Kaiso (ZBTB33) from human, mouse, rat and other species. Validated for IHC, IP and WB.
About ZBTB33 / Kaiso
ZBTB33 / Kaiso is a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. Q86T24 NM_006777 NP_006768.1

ZBTB33 Antibody, Kaiso Antibody, WUGSC:H_DJ525N14.1 Antibody, ZNF-kaiso Antibody, Kaiso transcription factor Antibody, ZNF348 Antibody


Specifications

Target
Mouse ZBTB33 / Kaiso
Host
Mouse
Reactivity
Mouse, Human, Rat, Dog, Hamster, Chicken (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC (1 µg/ml)
  • Western blot (0.5 - 2 µg/ml)
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ZBTB33 / Kaiso antibody was raised against recombinant N-terminal GST and His-tagged fusion protein corresponding to the central region of murine Kaiso (aa 103-584).
Epitope
aa103-584
Specificity
Recognizes Kaiso, Mr 97kD. Species cross-reactivity: Human, mouse, rat, canine, chicken, hamster and simian.
Usage
Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and Supershift. Immunohistochemistry: 1 ug/ml gives positive nuclear immunostaining for Kaiso in NIH 3T3 cells fixed with 100% methanol. Western Blot: 0.5-2 ug/ml detects Kaiso in RIPA lysates from 3T3/A31 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Kaiso (2 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 2-5 ug immunoprecipitates Kaiso from RIPA lysates from NIH 3T3 cells.1, 3. Supershift: Supershifts a specific methyl-CpG complex.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: United States
Date Requested: 8/18/2018

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