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Anti-ZBTB33 / Kaiso Antibody (aa103-584) LS-C16122

Ordering

Wt. Vol. Conc. Price
200 µg - - $530
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ZBTB33 / Kaiso Antibodies

Anti-ZBTB33 / Kaiso Antibody (N-Terminus) IHC-plus™ LS-B1116
Rabbit Polyclonal to Human ZBTB33 / Kaiso
Human, Monkey, Bovine, Dog, Guinea pig, Hamster, Horse, Pig
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-ZBTB33 / Kaiso Antibody LS-C71180
Mouse Monoclonal (IgG1) to Human ZBTB33 / Kaiso
Human, Mouse, Rat
Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Anti-ZBTB33 / Kaiso Antibody LS-C130320
Goat Polyclonal (IgG) to Human ZBTB33 / Kaiso
Human
Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG1) to Mouse ZBTB33 / Kaiso
Mouse, Human, Rat, Dog, Hamster, Chicken
IHC, Western blot, Immunoprecipitation
Unconjugated

Details

Mouse ZBTB33 / Kaiso
Mouse
Mouse, Human, Rat, Dog, Hamster, Chicken (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC (1 µg/ml)
  • Western blot (0.5 - 2 µg/ml)
  • Immunoprecipitation

Specificity and Use

ZBTB33 / Kaiso antibody was raised against recombinant N-terminal GST and His-tagged fusion protein corresponding to the central region of murine Kaiso (aa 103-584).
aa103-584
Recognizes Kaiso, Mr 97kD. Species cross-reactivity: Human, mouse, rat, canine, chicken, hamster and simian.
Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and Supershift. Immunohistochemistry: 1 ug/ml gives positive nuclear immunostaining for Kaiso in NIH 3T3 cells fixed with 100% methanol. Western Blot: 0.5-2 ug/ml detects Kaiso in RIPA lysates from 3T3/A31 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Kaiso (2 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 2-5 ug immunoprecipitates Kaiso from RIPA lysates from NIH 3T3 cells.1, 3. Supershift: Supershifts a specific methyl-CpG complex.

Packaging

0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About ZBTB33 / Kaiso

Q86T24 NM_006777 NP_006768.1

ZBTB33 Antibody, Kaiso Antibody, WUGSC:H_DJ525N14.1 Antibody, ZNF-kaiso Antibody, Kaiso transcription factor Antibody, ZNF348 Antibody

ZBTB33 / Kaiso is a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters.

Requested From: United States
Date Requested: 12/4/2016

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