Kaiso antibody LS-C16122 is an unconjugated mouse monoclonal antibody to Kaiso (ZBTB33) from human, mouse, rat and other species. Validated for IHC, IP and WB.
Mouse ZBTB33 / Kaiso
Mouse, Human, Rat, Dog, Hamster, Chicken
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- IHC (1 µg/ml)
- Western blot (0.5 - 2 µg/ml)
- (applications tested for the base form of this product only)
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ZBTB33 / Kaiso antibody was raised against recombinant N-terminal GST and His-tagged fusion protein corresponding to the central region of murine Kaiso (aa 103-584).
Recognizes Kaiso, Mr 97kD. Species cross-reactivity: Human, mouse, rat, canine, chicken, hamster and simian.
Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and Supershift. Immunohistochemistry: 1 ug/ml gives positive nuclear immunostaining for Kaiso in NIH 3T3 cells fixed with 100% methanol. Western Blot: 0.5-2 ug/ml detects Kaiso in RIPA lysates from 3T3/A31 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Kaiso (2 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 2-5 ug immunoprecipitates Kaiso from RIPA lysates from NIH 3T3 cells.1, 3. Supershift: Supershifts a specific methyl-CpG complex.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.