Mouse ZBTB33 / Kaiso

Mouse

Mouse, Human, Rat, Dog, Hamster, Chicken (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein G purified

Unmodified

Recombinant N-Terminus GST and His-tagged fusion protein corresponding to the central region of murine Kaiso (aa 103-584).

aa103-584

Recognizes Kaiso, Mr 97kD. Species cross-reactivity: Human, mouse, rat, canine, chicken, hamster and simian.

• IHC (1 µg/ml)
• Western blot (0.5 - 2 µg/ml)
• Immunoprecipitation

Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and Supershift. Immunohistochemistry: 1 ug/ml gives positive nuclear immunostaining for Kaiso in NIH 3T3 cells fixed with 100% methanol. Western Blot: 0.5-2 ug/ml detects Kaiso in RIPA lysates from 3T3/A31 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Kaiso (2 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 2-5 ug immunoprecipitates Kaiso from RIPA lysates from NIH 3T3 cells.1, 3. Supershift: Supershifts a specific methyl-CpG complex.

0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q86T24 NCBI: NM_006777 NP_006768.1