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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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YWHAQ / 14‑3‑3 Theta Antibody (aa41‑90) LS‑C119363
14-3-3 Theta antibody LS-C119363 is an unconjugated rabbit polyclonal antibody to 14-3-3 Theta (YWHAQ) from human, mouse and rat. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C119363-50
50 µl
Unavailable
LS-C119363-100
100 µl
Unavailable

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Immunohistochemical analysis of 14-3-3 theta/tau staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat, Bovine, Pig, Rabbit, Chicken
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Immunohistochemical analysis of 14-3-3 theta staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Western blot of Phospho-14-3-3 theta/beta (S232) antibody
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Product Description

14-3-3 Theta antibody LS-C119363 is an unconjugated rabbit polyclonal antibody to 14-3-3 Theta (YWHAQ) from human, mouse and rat. Validated for Peptide-ELISA and WB.
About YWHAQ / 14-3-3 Theta
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1. P27348 NM_006826 NP_006817.1

YWHAQ Antibody, 14-3-3 protein T-cell Antibody, 14-3-3 protein theta Antibody, 14-3-3 protein tau Antibody, 14-3-3 Antibody, 14-3-3 Tau Antibody, 1C5 Antibody, Protein HS1 Antibody, HS1 Antibody, Protein tau Antibody


Specifications

Target
Human YWHAQ / 14-3-3 Theta
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000)
Immunogen
Synthetic peptide from human 14-3-3 thet (aa41-90).
Epitope
aa41-90
Specificity
14-3-3 thet Antibody detects endogenous levels of total 14-3-3 thet protein.
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Store at -20°C for up to 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of extracts from HUVEC/HeLa cells, using 14-3-3 theta Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from HUVEC/HeLa cells, using 14-3-3 theta Antibody. The lane on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from HUVEC/HeLa cells, using 14-3-3 theta Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from HUVEC/HeLa cells, using 14-3-3 theta Antibody. The lane on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from HUVEC/HeLa cells, using 14-3-3 theta Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from HUVEC/HeLa cells, using 14-3-3 theta Antibody. The lane on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from HUVEC/HeLa cells, using 14-3-3 theta Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from HUVEC/HeLa cells, using 14-3-3 theta Antibody. The lane on the right is treated with the synthesized peptide.


Requested From: 
Date Requested: 6/23/2018

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