Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 40B7] (IgM) to Yeast Pma1p
Immunofluorescence, Western blot
Yeast (tested or 100% immunogen sequence identity)
IgM Monoclonal [40B7]
Tissue culture supernatant
Immunofluorescence (1:500 - 1:5000)
Western blot (1:1000 - 1:10000)
Specificity and Use
For western blots of yeast protein samples (cell lysates), try Yeast Pma1p antibody diluted 1:10000, followed by chemiluminescent detection (ECL). For non-ECL western detection methods, try the antibody diluted 1:1000-1:5000. For immunofluorescence on yeast cells, try the antibody diluted 1:500-1:5000. the antibody reacts with Pma1p based on the size of the band detected on western blots and the immunofluorescence localization pattern. the antibody also detects Pma1p-containing fusion proteins on western blots.
Tissue culture supernatant, 10 mM sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.