Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Yeast (tested or 100% immunogen sequence identity)
Protein A purified
Western blot (1:1000 - 1:10000)
ELISA (1:5000 - 1:25000)
Specificity and Use
Yeast Mer2 antibody was raised against synthetic peptide corresponding to amino acids 26-35 of Saccharomyces cerevisiae Mer2 protein.
the Saccharomyces cerevisiae Mer2 protein. Reactivity occurs against Saccharomyces cerevisiae Mer2 protein and reactivity is independent of phosphorylation at residue S30. A BLAST analysis was used to suggest minimal cross reactivity with Mer2 homologs from other sources
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kD in size corresponding to Mer2 protein by western blotting in the appropriate cell lysate or extract. This antibody is reactive with both phosphorylated and unphosphorylated Mer2 at the S30 position.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Anti-Mer2 pS30 Antibody - Western Blot. Western blot of affinity purified anti-S. cerevisiae Mer2 antibody shows detection of phosphorylated and unphosphorylated Mer2 in wild type, phosphatase treated and mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody is reactive with both phosphor-ylated and unphosphorylated Mer2 at the S30 position. The primary antibody was used at a 1:5000 dilution. Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.