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Anti-YAP / YAP1 Antibody (clone 2F12) IHC-plus™ LS-B4494

Note: This antibody replaces LS-C105100

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml Unavailable

Most Popular YAP / YAP1 Antibodies

Anti-YAP / YAP1 Antibody (clone 2F12) IHC-plus™ LS-B4494
Mouse Monoclonal [clone 2F12] (IgG2a,k) to Human YAP / YAP1
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-YAP / YAP1 Antibody (clone 2H1) IHC-plus™ LS-B4495
Mouse Monoclonal [clone 2H1] (IgG2a,k) to Human YAP / YAP1
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 2F12] (IgG2a,k) to Human YAP / YAP1
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human YAP / YAP1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [2F12]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (1 - 5 µg/ml)
  • ELISA

Specificity and Use

YAP / YAP1 antibody was raised against yAP1 (NP_006097, 53 a.a. ~ 162 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Western Blot (Cell lysate) - positive control Hela NE. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (30 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).

Packaging

PBS, pH 7.4.
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About YAP / YAP1

P46937 NM_006106 NP_006097.2

YAP1 Antibody, 65 kDa Yes-associated protein Antibody, YAP Antibody, Yes-associated protein beta Antibody, Yes-associated protein delta Antibody, Yorkie homolog Antibody, YAP2 Antibody, Yes-associated protein 1 Antibody, Yes-associated protein 2 Antibody, YKI Antibody, YAP65 Antibody

Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis.

Immunohistochemistry

Immunohistochemistry
Anti-YAP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4494 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-YAP1 antibody IHC of human colon, muscularis mucosa. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4494 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-YAP1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4494 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to YAP1 on HeLa cell (antibody concentration 30 ug/ml).

Western blot

Western blot
Western blot of YAP1 expression in transfected 293T cell line by YAP1 monoclonal antibody clone 2F12.

Western blot

Western blot
YAP1 monoclonal antibody clone 2F12 Western blot of YAP1 expression in HeLa NE.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged YAP1 is approximately 0.03 ng/ml as a capture antibody.
 

Requested From: 
Date Requested: 3/23/2017

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