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Anti-XRCC6 / Ku70 Antibody (aa228-254) LS-C160674

Catalog Size Price
LS-C160674-400 400 µl Unavailable

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100% Guaranteed
Rabbit Polyclonal to Human XRCC6 / Ku70
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human XRCC6 / Ku70
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

LS-E7730 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C160674. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa228-254
This XRCC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-254 amino acids from the Central region of human XRCC6.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About XRCC6 / Ku70

P12956 NM_001469 NP_001460.1

XRCC6 Antibody, 5-dRP lyase Ku70 Antibody, 70 kDa subunit of Ku antigen Antibody, CTCBF Antibody, DNA repair protein XRCC6 Antibody, D22S671 Antibody, D22S731 Antibody, G22P1 Antibody, Ku autoantigen, 70kDa Antibody, Ku autoantigen p70 subunit Antibody, Thyroid-lupus autoantigen p70 Antibody, TLAA Antibody, CTC75 Antibody, KU70 Antibody, ML8 Antibody, Thyroid-lupus autoantigen Antibody

Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination.

Immunohistochemistry

Immunohistochemistry
XRCC6 antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Immunofluorescence
Confocal immunofluorescence of XRCC6 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot
XRCC6 Antibody western blot of 293,A549 cell line lysates (35 ug/lane). The XRCC6 antibody detected the XRCC6 protein (arrow).

Flow Cytometry

Flow Cytometry
XRCC6 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: 
Date Requested: 8/20/2017

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