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Anti-XPOT / Exportin-T Antibody (aa362-411) LS-C80366

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular XPOT / Exportin-T Antibodies

Anti-XPOT / Exportin-T Antibody (aa362-411) LS-C80366
Rabbit Polyclonal (IgG) to Human XPOT / Exportin-T
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken
Western blot
Unconjugated
Western blot Image
Anti-XPOT / Exportin-T Antibody (aa825-875) IHC-plus™ LS-B13541
Rabbit Polyclonal to Human XPOT / Exportin-T
Human, Mouse, Bovine, Dog, Horse, Rabbit, Xenopus
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry - Paraffin Image
Anti-XPOT / Exportin-T Antibody (aa912-962) IHC-plus™ LS-B13542
Rabbit Polyclonal to Human XPOT / Exportin-T
Human, Mouse, Rat, Bovine, Dog, Chicken, Xenopus
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal (IgG) to Human XPOT / Exportin-T
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human XPOT / Exportin-T
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Xenopus, Zebrafish (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

XPOT / Exportin-T antibody was raised against synthetic peptide located between aa362-411 of human XPOT (O43592, NP_009166). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Turkey, Chicken, Lizard (100%); Panda, Platypus, Xenopus, Stickleback, Zebrafish (92%).
aa362-411
Human XPOT
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

Publications (1)

1
RAN nucleo-cytoplasmic transport and mitotic spindle assembly partners XPO7 and TPX2 are new prognostic biomarkers in serous epithelial ovarian cancer. C[Character e1]ceres-Gorriti KY, Carmona E, Barr[Character e8]s V, Rahimi K, L[Character e9]tourneau IJ, Tonin PN, Provencher D, Mes-Masson AM. PloS one. 2014 9:e91000. [Full Text Article] [PubMed:24625450] [PMC:PMC3953127]

About XPOT / Exportin-T

O43592 NM_007235 NP_009166.2

XPOT Antibody, Exportin(tRNA) Antibody, Exportin-T Antibody, TRNA exportin Antibody, XPO3 Antibody, Exportin, tRNA Antibody

Mediates the nuclear export of aminoacylated tRNAs. In the nucleus binds to tRNA and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the tRNA from the export receptor.

Western blot

Western blot
Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/11/2016

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