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Antibodies > XDH / Xanthine Oxidase > LS-C662123
 
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Catalog Number Size Price
LS-C662123-10 10 µg $318 
LS-C662123-100 100 µg $470 
XDH / Xanthine Oxidase Antibody - IHC analysis of Xanthine Oxidase using anti-Xanthine Oxidase antibody. Xanthine Oxidase was detected in paraffin-embedded section of rat intestine tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Xanthine Oxidase Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
XDH / Xanthine Oxidase Antibody - IHC analysis of Xanthine Oxidase using anti-Xanthine Oxidase antibody. Xanthine Oxidase was detected in paraffin-embedded section of mouse intestine tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Xanthine Oxidase Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
XDH / Xanthine Oxidase Antibody - Western blot analysis of Xanthine Oxidase using anti-Xanthine Oxidase antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysate,Lane 2: mouse liver tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Xanthine Oxidase antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Xanthine Oxidase at approximately 146KD. The expected band size for Xanthine Oxidase is at 146KD.
XDH / Xanthine Oxidase Antibody - Flow Cytometry analysis of A431 cells using anti-Xanthine Oxidase antibody. Overlay histogram showing A431 cells stained with anti-Xanthine Oxidase antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Xanthine Oxidase Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
XDH / Xanthine Oxidase Antibody - IHC analysis of Xanthine Oxidase using anti-Xanthine Oxidase antibody. Xanthine Oxidase was detected in paraffin-embedded section of rat intestine tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Xanthine Oxidase Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
XDH / Xanthine Oxidase Antibody - IHC analysis of Xanthine Oxidase using anti-Xanthine Oxidase antibody. Xanthine Oxidase was detected in paraffin-embedded section of mouse intestine tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Xanthine Oxidase Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
XDH / Xanthine Oxidase Antibody - Western blot analysis of Xanthine Oxidase using anti-Xanthine Oxidase antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysate,Lane 2: mouse liver tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Xanthine Oxidase antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Xanthine Oxidase at approximately 146KD. The expected band size for Xanthine Oxidase is at 146KD.
XDH / Xanthine Oxidase Antibody - Flow Cytometry analysis of A431 cells using anti-Xanthine Oxidase antibody. Overlay histogram showing A431 cells stained with anti-Xanthine Oxidase antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Xanthine Oxidase Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human XDH / Xanthine Oxidase Antibody (IHC, WB) LS‑C662123

Polyclonal Rabbit anti‑Human XDH / Xanthine Oxidase Antibody (IHC, WB) LS‑C662123

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Antibody:
XDH / Xanthine Oxidase Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662123-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
XDH / Xanthine Oxidase Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Xanthine Oxidase antibody LS-C662123 is an unconjugated rabbit polyclonal antibody to human Xanthine Oxidase (XDH). Validated for IHC and WB.
Target
Human XDH / Xanthine Oxidase
Synonyms
XDH | Xanthene dehydrogenase | XOR | Xanthine dehydrogenase/oxidase | Xanthine oxidase | XO | Xanthine dehydrogenase | Xanthine oxidoreductase | XDHA
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human Xanthine Oxidase recombinant protein (Position: T2-K343). Human Xanthine Oxidase shares 86.8% and 89.2% amino acid (aa) sequence identity with mouse and rat Xanthine Oxidase, respectively.
Specificity
Detected in milk (at protein level). .
Applications
  • IHC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About XDH / Xanthine Oxidase
P47989 NM_000379 NP_000370.2

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Featured Products

Product
XDH / Xanthine Oxidase Antibody (aa206‑234, APC) LS‑C273685
Species: Human, Mouse
Applications: IHC, Western blot, Flow Cytometry, ELISA
Product
XDH / Xanthine Oxidase Antibody (aa206‑234) LS‑C326386
Species: Human, Mouse
Applications: IHC, Western blot, Flow Cytometry, ELISA
XDH / Xanthine Oxidase Antibody - 1:100 staining human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
XDH / Xanthine Oxidase Antibody LS‑C807005
Species: Human, Rat
Applications: IHC, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Product
XDH / Xanthine Oxidase Antibody (Lyophilized) LS‑C209646
Species: Bovine
Applications: Western blot, Immunoprecipitation, ELISA
XDH / Xanthine Oxidase Antibody - Western Blot; Sample: Bovine Milk; Primary Ab: 5µg/ml Rabbit Anti-Human XDH Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
XDH / Xanthine Oxidase Antibody (aa1‑300) LS‑C372996
Species: Human, Bovine, Pig
Applications: Western blot
XDH / Xanthine Oxidase Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY XDH. (Right lane) cDNA for 48 hrs and lysed
XDH / Xanthine Oxidase Antibody (aa1‑280, clone OTI5D8) LS‑C796453
Species: Human
Applications: Western blot
See all XDH / Xanthine Oxidase Antibodies

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/26/2024
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