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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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WNT3A Antibody (Biotin) LS‑C104698
Note: This antibody replaces LS-C188026
Antibody
WNT3A antibody LS-C104698 is a biotin-conjugated rabbit polyclonal antibody to human WNT3A. Validated for ELISA and WB.
Catalog
Size
Price
LS-C104698-25
25 µg (1 mg/ml)
Unavailable
LS-C104698-50
50 µg
Unavailable

Popular WNT3A Products

Antibody
Species: Human, Mouse
Applications: Western blot, Immunoprecipitation
The lysates of A549 (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Wnt3a (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Species: Human
Applications: Western blot, ELISA
Anti-WNT3A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5876 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Guinea pig, Rabbit, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Anti-WNT3A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5896 concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human, Monkey, Rat, Bovine, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot

Product Description

WNT3A antibody LS-C104698 is a biotin-conjugated rabbit polyclonal antibody to human WNT3A. Validated for ELISA and WB.
About WNT3A
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. P56704 NM_033131 NP_149122.1

WNT3A Antibody, Protein Wnt-3a Antibody


Specifications

Target
Human WNT3A
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (1 µg/ml)
Immunogen
WNT3A antibody was raised against e.coli derived recombinant Human WNT-3a.
Specificity
Human WNT-3a
Usage
ELISA: To detect hWNT-3a by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hWNT-3a. To detect hWNT-3a by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human WNT-3a (LS-B8210) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hWNT-3a. Western Blot: To detect hWNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
25 µl or 50 µl Sterile PBS containing 0.1% BSA
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/19/2018

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