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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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WNT16 Antibody (aa236‑265) LS‑C160629
WNT16 antibody LS-C160629 is an unconjugated rabbit polyclonal antibody to human WNT16. Validated for IHC and WB.
Catalog
Size
Price
LS-C160629-400
400 µl
Unavailable

Popular WNT16 Products

Anti-WNT16 antibody LS-A9629 IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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Anti-WNT16 antibody LS-A9630 IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Anti-WNT16 antibody LS-A9629 IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Mouse, Rat, Hamster
Applications: IHC, IHC - Paraffin

Product Description

WNT16 antibody LS-C160629 is an unconjugated rabbit polyclonal antibody to human WNT16. Validated for IHC and WB.
About WNT16
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It contains two transcript variants diverging at the 5' termini. These two variants are proposed to be the products of separate promoters and not to be splice variants from a single promoter. Q9UBV4 NM_016087 NP_057171.2

WNT16 Antibody, Protein Wnt-16 Antibody


Specifications

Target
Human WNT16
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E2282 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C160629. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa236-265
Specificity
This WNT16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-265 amino acids from the C-terminal region of human WNT16.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

WNT16 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
WNT16 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of lysates from human brain, mouse brain tissue (from left to right), using WNT16 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Western blot of lysates from human brain, mouse brain tissue (from left to right), using WNT16 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

Western blot

WNT16 Antibody western blot of CEM cell line lysates (35 ug/lane). The WNT16 antibody detected the WNT16 protein (arrow).
WNT16 Antibody western blot of CEM cell line lysates (35 ug/lane). The WNT16 antibody detected the WNT16 protein (arrow).

Immunohistochemistry

WNT16 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
WNT16 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of lysates from human brain, mouse brain tissue (from left to right), using WNT16 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Western blot of lysates from human brain, mouse brain tissue (from left to right), using WNT16 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

Western blot

WNT16 Antibody western blot of CEM cell line lysates (35 ug/lane). The WNT16 antibody detected the WNT16 protein (arrow).
WNT16 Antibody western blot of CEM cell line lysates (35 ug/lane). The WNT16 antibody detected the WNT16 protein (arrow).

Immunohistochemistry

WNT16 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
WNT16 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of lysates from human brain, mouse brain tissue (from left to right), using WNT16 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Western blot of lysates from human brain, mouse brain tissue (from left to right), using WNT16 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

Western blot

WNT16 Antibody western blot of CEM cell line lysates (35 ug/lane). The WNT16 antibody detected the WNT16 protein (arrow).
WNT16 Antibody western blot of CEM cell line lysates (35 ug/lane). The WNT16 antibody detected the WNT16 protein (arrow).

Immunohistochemistry

WNT16 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
WNT16 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of lysates from human brain, mouse brain tissue (from left to right), using WNT16 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Western blot of lysates from human brain, mouse brain tissue (from left to right), using WNT16 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

Western blot

WNT16 Antibody western blot of CEM cell line lysates (35 ug/lane). The WNT16 antibody detected the WNT16 protein (arrow).
WNT16 Antibody western blot of CEM cell line lysates (35 ug/lane). The WNT16 antibody detected the WNT16 protein (arrow).


Requested From: 
Date Requested: 6/17/2018

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