Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 345712] (IgG3) to West Nile Virus
West Nile Virus
Western blot, Immunoprecipitation, ELISA, Neutralization
West Nile Virus
West Nile Virus (tested or 100% immunogen sequence identity)
IgG3 Monoclonal 
Protein G purified
Specificity and Use
West Nile Virus antibody was raised against e. coli-derived rwnvNS3/NS2b.
Recognizes West Nile Virus NS2b subunit.
Western Blot, Neutralization, Immunoprecipitation, Direct ELISA. Western Blot: This antibody can be used at 1-2 ug/mL with the appropriate secondary reagents to detect west nile virus NS3/NS2b complex. Using a colorimetric detection system, the detection limit for rwnvNS3/NS2b complex is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Neutralization of west nile virus NS3 bioactivity: This antibody has been used to inhibit/neutralize the enzymatic activity of rwnvNS3/NS2b. The antibody was preincubated with the enzyme at different molar ratios at room temperature for 30 min. The enzyme was then assayed with a peptide substrate, Ac-REKR-AFC. 50% of the proteolytic activity was inhibited by the antibody at approximately 13 ug/mL (IC50) under conditions in which the enzyme was present at 10 ug/mL and the substrate concentration was 200uM. Considering the molecular masses of the enzyme (27kD) and the antibody (150kD), IC50 was achieved at approximately 1:4 molar ratio of the antibody to the enzyme. Immunoprecipitation: This antibody was used at a concentration of 25 ug/mL to immunoprecipitate rwnvNS3/NS2b from conditioned cell culture medium. The recovered rwnvNS3/NS2b can be detected in Western blots using a polyclonal antibody. Direct ELISA: This antibody can be used at 0.5-1.0 ug/mL with the appropriate secondary reagents to detect west nile virus NS3/NS2b complex. The detection limit for rwnvNS3/NS2b is approximately 1 ng/well.
Lyophilized from TBS, 5% trehalose. Reconstitution: Reconstitute, sterile TBS. If 1 ml of TBS is used, the antibody concentration will be 100 ug/ml.