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Anti-WC1 / ATP7B Antibody (N-Terminus) IHC-plus™ LS-B2302

Note: This antibody replaces LS-C2764, LS-C74359

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Most Popular WC1 / ATP7B Antibodies

Anti-WC1 / ATP7B Antibody (N-Terminus) IHC-plus™ LS-B2302
Rabbit Polyclonal to Human WC1 / ATP7B
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-WC1 / ATP7B Antibody (aa1361-1391) LS-C99220
Rabbit Polyclonal to Human WC1 / ATP7B
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Human WC1 / ATP7B
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated

Details

Human WC1 / ATP7B
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:1000)

Specificity and Use

WC1 / ATP7B antibody was raised against a synthetic peptide made to an internal sequence near the N-terminus of human ATP7b
N-Terminus
ATP7b.
Immunohistochemistry: LS-B2302 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2302 was determined to be 5 ug/ml.

Packaging

Tris-citrate/phosphate buffer, 0.1% sodium azide.
Store at 4°C. Do not freeze.
For research use only.

Publications (1)

1
Predictive and prognostic value of human copper transporter 1 (hCtr1) in patients with stage III non-small-cell lung cancer receiving first-line platinum-based doublet chemotherapy. Chen HH, Yan JJ, Chen WC, Kuo MT, Lai YH, Lai WW, Liu HS, Su WC. Lung cancer (Amsterdam, Netherlands). 2012 Feb;75:228-34. [Full Text Article] [PubMed:21788094] [PMC:PMC3319119]

About WC1 / ATP7B

P35670 NM_000053 NP_000044.2

ATP7B Antibody, Copper-transporting ATPase 2 Antibody, WC1 Antibody, Wilson disease Antibody, PWD Antibody, WD Antibody, Copper pump 2 Antibody, WND Antibody

WC1 / ATP7B is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile.

Immunohistochemistry

Immunohistochemistry
Anti-ATP7B antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2302 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
ATP7b detected in 20 ug of mouse brain membrane fraction using LS-B2302. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/5/2016

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