Human VTN / Vitronectin

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b,k Monoclonal

HV2

Unconjugated

Protein A/G purified

Unmodified

Human vitronectin purified from plasma by heparin-affinity chromatography.

aa121-133

Highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). This antibody is human-specific (no reactivity with cat, dog, cow, sheep, goat, pig, rabbit, horse). The epitope is located in the connecting region. Binding can be competed selectively by a peptide comprising aa 121-133. Reactivity: Can be used to purify vitronectin from human plasma by affinity chromatography. It can also be used to quantitatively affinity-deplete human plasma or serum of vitronectin. It partially denatures vitronectin upon antibody binding. It binds equally well to native and denatured vitronectin and can be used to quantitative vitronectin in human plasma in a sandwich ELISA with clone 6F211. It does not interfere with the binding of any known vitronectin ligands. Also binds to vitronectin in ELISA when vitronectin is coated directly onto the microtiter well.

• Western blot (1:100)
• ELISA (1:32000)

Suitable for use in ELISA, Western Blot. ELISA: 1:32000. Western Blot: 1:100.

PBS, pH 7.2, 15 mM Sodium Azide

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P04004 NCBI: NM_000638 NP_000629.3