LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
VPS35 Antibody (aa783‑796) IHC‑plus™ LS‑B5909
Note: This antibody replaces LS-C55677
VPS35 antibody LS-B5909 is an unconjugated goat polyclonal antibody to VPS35 from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Catalog
Size
Price
LS-B5909-50
50 µg (0.5 mg/ml)
Unavailable

Popular VPS35 Products

Antibody
Species: Human, Mouse, Rat
Applications: IHC, Western blot
VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm and membrane on colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. VPS35 antibody [C3], C-term dilution:1:250.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot
Western blot analysis of extracts of various cell lines.
Species: Human, Mouse, Rat
Applications: IHC, Western blot
Western blot of Vps35 Antibody - C-terminal region in Mouse Lung cells lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human, Rat, Bovine, Dog, Horse, Pig, Rabbit
Applications: Western blot

Product Description

VPS35 antibody LS-B5909 is an unconjugated goat polyclonal antibody to VPS35 from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
About VPS35
VPS35 belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Q96QK1 NM_018206 NP_060676.2

VPS35 Antibody, MEM3 Antibody, Maternal-embryonic 3 Antibody, Vesicle protein sorting 35 Antibody, PARK17 Antibody, HVPS35 Antibody


Specifications

Target
Human VPS35
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Hamster, Horse, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Bat, Dog, Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (0.01 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
VPS35 antibody was raised against synthetic peptide C-SPESEGPIYEGLIL from the C-terminus of human VPS35 (NP_060676.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Horse, Rabbit (100%); Dog, Bat, Opossum, Turkey, Chicken, Platypus (93%); Stickleback, Pufferfish, Zebrafish (86%).
Blocking Peptide
LS-E25680 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5909. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa783-796
Specificity
Human VPS35. Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (1)

1
Rab GTPase-activating proteins in autophagy: regulation of endocytic and autophagy pathways by direct binding to human ATG8 modifiers. Popovic D, Akutsu M, Novak I, Harper JW, Behrends C, Dikic I. Molecular and cellular biology. 2012 32:1733-44. (WB; Human) [Full Text Article] [PubMed:22354992] [PMC:PMC3347240]

Customer Reviews (0)


Images


Immunohistochemistry

Anti-VPS35 antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5909 concentration 3.75 ug/ml.
Anti-VPS35 antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5909 concentration 3.75 ug/ml.

Immunofluorescence

Staining of PFA-fixed and saponin permeabilized HEK293 and detected with FITC in confocal microscopy. Data obtained from Aarhus University Denmark.
Staining of PFA-fixed and saponin permeabilized HEK293 and detected with FITC in confocal microscopy. Data obtained from Aarhus University Denmark.

Immunofluorescence

Staining of PFA-fixed and saponin permeabilized SHSY5Y and detected with FITC in confocal microscopy. Data obtained from Dr. M. Schallburg Nielsen, Aarhus University Denmark.
Staining of PFA-fixed and saponin permeabilized SHSY5Y and detected with FITC in confocal microscopy. Data obtained from Dr. M. Schallburg Nielsen, Aarhus University Denmark.

Western blot

Staining (0.05 ug/ml) of human brain cerebellum lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.
Staining (0.05 ug/ml) of human brain cerebellum lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.

Immunohistochemistry

Anti-VPS35 antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5909 concentration 3.75 ug/ml.
Anti-VPS35 antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5909 concentration 3.75 ug/ml.

Immunofluorescence

Staining of PFA-fixed and saponin permeabilized HEK293 and detected with FITC in confocal microscopy. Data obtained from Aarhus University Denmark.
Staining of PFA-fixed and saponin permeabilized HEK293 and detected with FITC in confocal microscopy. Data obtained from Aarhus University Denmark.

Immunofluorescence

Staining of PFA-fixed and saponin permeabilized SHSY5Y and detected with FITC in confocal microscopy. Data obtained from Dr. M. Schallburg Nielsen, Aarhus University Denmark.
Staining of PFA-fixed and saponin permeabilized SHSY5Y and detected with FITC in confocal microscopy. Data obtained from Dr. M. Schallburg Nielsen, Aarhus University Denmark.

Western blot

Staining (0.05 ug/ml) of human brain cerebellum lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.
Staining (0.05 ug/ml) of human brain cerebellum lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.

Immunohistochemistry

Anti-VPS35 antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5909 concentration 3.75 ug/ml.
Anti-VPS35 antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5909 concentration 3.75 ug/ml.

Immunofluorescence

Staining of PFA-fixed and saponin permeabilized HEK293 and detected with FITC in confocal microscopy. Data obtained from Aarhus University Denmark.
Staining of PFA-fixed and saponin permeabilized HEK293 and detected with FITC in confocal microscopy. Data obtained from Aarhus University Denmark.

Immunofluorescence

Staining of PFA-fixed and saponin permeabilized SHSY5Y and detected with FITC in confocal microscopy. Data obtained from Dr. M. Schallburg Nielsen, Aarhus University Denmark.
Staining of PFA-fixed and saponin permeabilized SHSY5Y and detected with FITC in confocal microscopy. Data obtained from Dr. M. Schallburg Nielsen, Aarhus University Denmark.

Western blot

Staining (0.05 ug/ml) of human brain cerebellum lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.
Staining (0.05 ug/ml) of human brain cerebellum lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.

Immunohistochemistry

Anti-VPS35 antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5909 concentration 3.75 ug/ml.
Anti-VPS35 antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5909 concentration 3.75 ug/ml.

Immunofluorescence

Staining of PFA-fixed and saponin permeabilized HEK293 and detected with FITC in confocal microscopy. Data obtained from Aarhus University Denmark.
Staining of PFA-fixed and saponin permeabilized HEK293 and detected with FITC in confocal microscopy. Data obtained from Aarhus University Denmark.

Immunofluorescence

Staining of PFA-fixed and saponin permeabilized SHSY5Y and detected with FITC in confocal microscopy. Data obtained from Dr. M. Schallburg Nielsen, Aarhus University Denmark.
Staining of PFA-fixed and saponin permeabilized SHSY5Y and detected with FITC in confocal microscopy. Data obtained from Dr. M. Schallburg Nielsen, Aarhus University Denmark.

Western blot

Staining (0.05 ug/ml) of human brain cerebellum lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.
Staining (0.05 ug/ml) of human brain cerebellum lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.


Requested From: 
Date Requested: 6/25/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number