LSBio
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA and Assay Kits
  • All Kits
  • Assay Kits
  • Traditional ELISA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Phospho-Specific ELISA Kits
  • ELISA Development Kits
  • Chemiluminescent CLIA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Biochemicals
  • All Biochemicals

  • Immunohistochemistry
  • Immunohistochemistry Reagents
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Reviews
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Protocols
  • Secure Logins
  • Login to Our Secure ShareFile Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login ▾
Wish List
View Cart

Anti-VPS35 Antibody (aa783-796) IHC-plus™ LS-B5909

Note: This antibody replaces LS-C55677
Catalog Size Price
LS-B5909-50 50 µg (0.5 mg/ml) Unavailable

Related Products

9 VPS35 Antibodies

Most Popular VPS35 Antibodies

Anti-VPS35 Antibody (aa190-240) LS-C95258
Rabbit Polyclonal (IgG) to Human VPS35
Human, Mouse, Rat
IHC, Western blot
Unconjugated
Anti-VPS35 Antibody (aa783-796) IHC-plus™ LS-B5909
Goat Polyclonal to Human VPS35
Human, Monkey, Mouse, Rat, Bovine, Hamster, Horse, Rabbit
IHC - Paraffin, Western blot, ELISA
Unconjugated
Anti-VPS35 antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5909 concentration 3.75 ug/ml.
Anti-VPS35 Antibody (aa733-796) LS-C185941
Rabbit Polyclonal to Human VPS35
Human
IHC - Paraffin, ICC, Western blot
Unconjugated
VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm and membrane on colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. VPS35 antibody [C3], C-term dilution:1:250.
Anti-VPS35 Antibody LS-C409122
Rabbit Polyclonal (IgG) to Human VPS35
Human, Mouse, Rat
IHC, Western blot
Unconjugated
Western blot analysis of extracts of various cell lines.
Anti-VPS35 Antibody (C-Terminus, HRP) LS-C463013
Rabbit Polyclonal to Mouse VPS35
Mouse, Human, Rat, Bovine, Dog, Horse, Pig, Rabbit
Western blot
HRP Conjugated
Western blot of Vps35 Antibody - C-terminal region in Mouse Lung cells lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

100% Guaranteed Publications (1)
Goat Polyclonal to Human VPS35
Human, Monkey, Mouse, Rat, Bovine, Hamster, Horse, Rabbit
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human VPS35
Goat
Human, Monkey, Mouse, Rat, Bovine, Hamster, Horse, Rabbit (tested or 100% immunogen sequence identity)
Bat, Dog, Chicken (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (0.01 - 1 µg/ml)
  • ELISA (1:32000)

Specificity and Use

VPS35 antibody was raised against synthetic peptide C-SPESEGPIYEGLIL from the C-terminus of human VPS35 (NP_060676.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Horse, Rabbit (100%); Dog, Bat, Opossum, Turkey, Chicken, Platypus (93%); Stickleback, Pufferfish, Zebrafish (86%).
LS-E25680 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5909. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa783-796
Human VPS35. Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

Publications (1)

1
Rab GTPase-activating proteins in autophagy: regulation of endocytic and autophagy pathways by direct binding to human ATG8 modifiers. Popovic D, Akutsu M, Novak I, Harper JW, Behrends C, Dikic I. Molecular and cellular biology. 2012 32:1733-44. (WB; Human) [Full Text Article] [PubMed:22354992] [PMC:PMC3347240]

About VPS35

Q96QK1 NM_018206 NP_060676.2

VPS35 Antibody, MEM3 Antibody, Maternal-embryonic 3 Antibody, Vesicle protein sorting 35 Antibody, PARK17 Antibody, HVPS35 Antibody

VPS35 belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function.

Immunohistochemistry

Anti-VPS35 antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5909 concentration 3.75 ug/ml.
Anti-VPS35 antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5909 concentration 3.75 ug/ml.

Immunofluorescence

Staining of PFA-fixed and saponin permeabilized HEK293 and detected with FITC in confocal microscopy. Data obtained from Aarhus University Denmark.
Staining of PFA-fixed and saponin permeabilized HEK293 and detected with FITC in confocal microscopy. Data obtained from Aarhus University Denmark.

Immunofluorescence

Staining of PFA-fixed and saponin permeabilized SHSY5Y and detected with FITC in confocal microscopy. Data obtained from Dr. M. Schallburg Nielsen, Aarhus University Denmark.
Staining of PFA-fixed and saponin permeabilized SHSY5Y and detected with FITC in confocal microscopy. Data obtained from Dr. M. Schallburg Nielsen, Aarhus University Denmark.

Western blot

Staining (0.05 ug/ml) of human brain cerebellum lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.
Staining (0.05 ug/ml) of human brain cerebellum lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.

Requested From: 
Date Requested: 4/20/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number