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Anti-Vitamin D Receptor / VDR Antibody (aa89-105) IHC-plus™ LS-B2577

Note: This antibody replaces LS-C11024

Ordering

Wt. Vol. Conc. Price
- 250 µl 0.2 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Vitamin D Receptor / VDR Antibodies

Anti-Vitamin D Receptor / VDR Antibody (aa274-299, PE) LS-C273294
Rabbit Polyclonal (IgG) to Human Vitamin D Receptor / VDR
Human
Western blot, Flow Cytometry, ELISA
PE Conjugated
Anti-Vitamin D Receptor / VDR Antibody (clone 2F4) IHC-plus™ LS-B4449
Mouse Monoclonal [clone 2F4] (IgG2a,k) to Human Vitamin D Receptor / VDR
Human
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-Vitamin D Receptor / VDR Antibody (aa89-105) IHC-plus™ LS-B2577
Rat Monoclonal (IgG2b) to Chicken Vitamin D Receptor / VDR
Chicken, Human, Mouse, Rat, Hamster, Pig, Frog
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rat Monoclonal (IgG2b) to Chicken Vitamin D Receptor / VDR
Chicken, Human, Mouse, Rat, Hamster, Pig, Frog
IHC - Paraffin, Western blot
Unconjugated

Details

Chicken Vitamin D Receptor / VDR
Rat
Chicken, Human, Mouse, Rat, Hamster, Pig, Frog (tested or 100% immunogen sequence identity)
IgG2b Monoclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 - 2 µg/ml)

Specificity and Use

Vitamin D Receptor / VDR antibody was raised against vitamin D receptor protein from chicken intestine (53kD). Cellular Localization: Nuclear. Epitope: 89-105.
aa89-105
Recognizes both the occupied and unoccupied forms of VDR. Does not cross-react with estrogen and glucocorticoid receptors. In mammals, recognizes a protein of ~53kD which is identified as vitamin D Receptor (VDR). In avians, VDR appears as a doublet of 58 and 60kD. Species cross-reactivity: Human, porcine, mouse, rat, hamster, frog, chicken and fish.
Immunohistochemistry: LS-B2577 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2577 was determined to be 10 ug/ml. Positive control: LNCaP and HeLa cells.

Packaging

PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About Vitamin D Receptor / VDR

P11473 NM_000376 NP_000367.1

VDR Antibody, NR1I1 Antibody, Vitamin D3 receptor Antibody, Vitamin D receptor Antibody

The vitamin D receptor, a NR1 Thyroid Hormone-Like Receptor, is activated by calcitriol, the active hormonal form of vitamin D. The activated receptor binds DNA at a specific vitamin D-responsive element and promotes transcription of vitamin D target genes such as osteocalcin, the most abundant noncollagenous protein in bone. The vitamin D receptor affects growth, bone formation, and female reproduction.

Immunohistochemistry

Immunohistochemistry
Anti-VDR antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2577 concentration 10 ug/ml.
 

Requested From: United States
Date Requested: 12/10/2016

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