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Anti-VDAC1 / PORIN Antibody (aa185-197) IHC-plus™ LS-B435

Note: This antibody replaces LS-C18921, LS-C47598, LS-C11021

Ordering

Wt. Vol. Conc. Price
50 µg - 0.69 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular VDAC1 / PORIN Antibodies

Anti-VDAC1 / PORIN Antibody (aa185-197) IHC-plus™ LS-B435
Rabbit Polyclonal to Human VDAC1 / PORIN
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-VDAC1 / PORIN Antibody (C-Terminus) IHC-plus™ LS-B7906
Rabbit Polyclonal (IgG) to Human VDAC1 / PORIN
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-VDAC1 / PORIN Antibody (aa1-283, clone S152B-23) LS-C209548
Mouse Monoclonal [clone S152B-23] (IgG2a) to Human VDAC1 / PORIN
Human, Mouse, Rat
IHC, ICC, Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human VDAC1 / PORIN
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human VDAC1 / PORIN
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:10000 - 1:40000)

Specificity and Use

VDAC1 / PORIN antibody was raised against synthetic peptide from human VDAC1.
aa185-197
Amino acids 185-197 of Human VDAC1/Porin1.
Immunohistochemistry: LS-B435 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B435 was determined to be 2.5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About VDAC1 / PORIN

P21796 NM_003374 NP_003365.1

VDAC1 Antibody, HVDAC1 Antibody, Plasmalemmal porin Antibody, Porin 31HM Antibody, PORIN Antibody, PORIN-31-HL Antibody, Vdac5 Antibody, VDAC Antibody, Mitochondrial porin Antibody, Porin 31HL Antibody, VDAC-1 Antibody

Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective.

Immunohistochemistry

Immunohistochemistry
Anti-VDAC1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B435 concentration 5 ug/ml.

Western blot

Western blot
Anti-VDAC/Porin Antibody - Western Blot. Western blot of Affinity Purified anti-VDAC/Porin antibody detects a single band at ~32 kD corresponding to VDAC/Porin (arrowhead) present in a rat heart whole cell lysate. Approximately 35 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1200 at 4C overnight. Washes were followed by reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MXHu ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Requested From: United States
Date Requested: 12/8/2016

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