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Anti-VCAM1 / CD106 Antibody LS-C5801

Ordering

Wt. Vol. Conc. Price
100 µg - - $605
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular VCAM1 / CD106 Antibodies

Anti-VCAM1 / CD106 Antibody (aa25-698) LS-C150269
Goat Polyclonal (IgG) to Mouse VCAM1 / CD106
Mouse
IHC, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-VCAM1 / CD106 Antibody (clone 3H10) LS-C172657
Mouse Monoclonal [clone 3H10] (IgG1) to Human VCAM1 / CD106
Human, Monkey, Dog
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG1) to Human VCAM1 / CD106
Human
IHC - Frozen, Immunoprecipitation, Flow Cytometry
Unconjugated

Details

Human VCAM1 / CD106
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Unconjugated
Purified
Unmodified

Applications

  • IHC - Frozen
  • Immunoprecipitation
  • Flow Cytometry (1:10)

Specificity and Use

Reacts with human VCAM-1.
Useful for staining of VCAM-1 expressing endothelial cells. It permits staining of in vitro cultured cells and frozen tissue sections. Furthermore the antibody can be used for Flow Cytometry, immunoassays and Immunoprecipitation. Flow Cytometry: 1:10. Immunohistology: Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. In vitro cultured cells can be fixed with 1% paraformaldehyde, tissue sections are advised to be fixed for 10 min in pure acetone and followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised to be followed by a biotin conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1 ng/ml), IL-1 (100U/ml) or LPS (1 ug/ml) in tissue culture medium at 37?C. As negative control it is advised to use a control murine IgG1 antibody.

Packaging

PBS, 0.02% sodium azide, 0.1% BSA.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About VCAM1 / CD106

P19320 NM_001078 NP_001069.1

VCAM1 Antibody, CD106 Antibody, CD106 antigen Antibody, INCAM-100 Antibody, V-CAM 1 Antibody, VCAM-1 Antibody

Vascular cell adhesion molecule 1, VCAM-1, is a surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of monocytes and lymphocytes. VCAM-1 and integrin alpha-4-beta-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization. Studies of heart development have shown that deficiencies of VCAM and in alpha-4 integrin also result in epicardial dissolution and subsequent myocardial thinning.

Requested From: United States
Date Requested: 12/2/2016

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