LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
VCAM1 / CD106 Antibody LS‑C5801
CD106 antibody LS-C5801 is an unconjugated mouse monoclonal antibody to human CD106 (VCAM1). Validated for Flow, IHC and IP.
Antibody
Catalog
Size
Price
LS-C5801-100
100 µg
$710

Popular VCAM1 / CD106 Products

Anti-VCAM1 antibody IHC of human colon, adipocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4161 dilution 1:200.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VCAM1 mouse monoclonal antibody.
Species: Human, Monkey, Dog
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Antibody
Species: Human
Applications: IHC, IHC - Frozen, Western blot, Flow Cytometry, ELISA
Flow cytometry of VCAM1 / VCAM-1 / CD106 antibody This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, Western blot, Flow Cytometry, ELISA, Functional Assay
Antibody
Species: Mouse
Applications: IHC, IHC - Frozen, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Functional Assay

Product Description

CD106 antibody LS-C5801 is an unconjugated mouse monoclonal antibody to human CD106 (VCAM1). Validated for Flow, IHC and IP.

Specifications

Target
Human VCAM1 / CD106
Synonyms
VCAM1, CD106, CD106 antigen, INCAM-100, V-CAM 1, VCAM-1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • Immunoprecipitation
  • Flow Cytometry (1:10)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Specificity
Reacts with human VCAM-1.
Usage
Useful for staining of VCAM-1 expressing endothelial cells. It permits staining of in vitro cultured cells and frozen tissue sections. Furthermore the antibody can be used for Flow Cytometry, immunoassays and Immunoprecipitation. Flow Cytometry: 1:10. Immunohistology: Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. In vitro cultured cells can be fixed with 1% paraformaldehyde, tissue sections are advised to be fixed for 10 min in pure acetone and followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised to be followed by a biotin conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1 ng/ml), IL-1 (100U/ml) or LPS (1 ug/ml) in tissue culture medium at 37?C. As negative control it is advised to use a control murine IgG1 antibody.
Presentation
PBS, 0.02% Sodium Azide, 0.1% BSA
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
About VCAM1 / CD106
Vascular cell adhesion molecule 1, VCAM-1, is a surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of monocytes and lymphocytes. VCAM-1 and integrin alpha-4-beta-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization. Studies of heart development have shown that deficiencies of VCAM and in alpha-4 integrin also result in epicardial dissolution and subsequent myocardial thinning. P19320 NM_001078 NP_001069.1


Publications (0)


Customer Reviews (0)


Requested From: United States
Date Requested: 10/19/2018

Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy