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Anti-VASP Antibody (phospho-Ser239, clone 16C2) LS-C10997

Ordering

Wt. Vol. Conc. Price
100 µg 1000 µl 0.1 mg/ml $635
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular VASP Antibodies

Anti-VASP Antibody (phospho-Ser238) LS-C117505
Rabbit Polyclonal (IgG) to Human VASP
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-VASP Antibody (phospho-Ser157) LS-C178002
Rabbit Polyclonal to Human VASP
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 16C2] (IgG1,k) to Human VASP
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse
ICC, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Unconjugated

Details

Human VASP
Mouse
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [16C2]
Unconjugated
Gel filtration
Unmodified

Applications

  • ICC
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry (2.4 µg/ml)
  • ELISA (0.05 µg/ml)

Specificity and Use

VASP antibody was raised against kLH-conjugated, synthetic phosphopeptide corresponding to amino acids 236-242 of human phospho-VASP (RKV[pS]KQE).
pSer239
Recognizes human phosphorylated VASP, Mr 50kD. Species cross-reactivity: mouse.
Suitable for use in ELISA, Western Blot, Immunocytochemistry, FACS and Immunoprecipitation. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunocytochemistry: An independent laboratory has reported that 3 ug/ml antibody showed positive immunostaining in SNP or 8pCPT-cGMP-treated human platelets, fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton-X. FACS: 2.4 ug/ml using fixed and permeabilized human platelets, as reported by an independent laboratory. Immunoprecipitation: Immunoprecipitates phosphorylated VASP from vanadate-treated A431 cells, as reported by an independent laboratory. ELISA: 0.05 ug/ml, as reported by an independent laboratory.

Packaging

Lyophilized powder from PBS, 0.1% sodium azide, PEG, sucrose.
1,000 µl Reconstitute with 1ml ddH2O
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About VASP

P50552 NM_003370 NP_003361.1

VASP Antibody

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein.

Requested From: United States
Date Requested: 12/11/2016

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