Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
VASP antibody was raised against kLH-conjugated, synthetic phosphopeptide corresponding to amino acids 236-242 of human phospho-VASP (RKV[pS]KQE).
Recognizes human phosphorylated VASP, Mr 50kD. Species cross-reactivity: mouse.
Suitable for use in ELISA, Western Blot, Immunocytochemistry, FACS and Immunoprecipitation. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunocytochemistry: An independent laboratory has reported that 3 ug/ml antibody showed positive immunostaining in SNP or 8pCPT-cGMP-treated human platelets, fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton-X. FACS: 2.4 ug/ml using fixed and permeabilized human platelets, as reported by an independent laboratory. Immunoprecipitation: Immunoprecipitates phosphorylated VASP from vanadate-treated A431 cells, as reported by an independent laboratory. ELISA: 0.05 ug/ml, as reported by an independent laboratory.
Lyophilized powder from PBS, 0.1% sodium azide, PEG, sucrose.
1,000 µl Reconstitute with 1ml ddH2O
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein.