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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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USP47 Antibody (aa635‑870) LS‑C185775 Best Seller
USP47 antibody LS-C185775 is an unconjugated rabbit polyclonal antibody to USP47 from human and mouse. Validated for ICC and WB.
Catalog
Size
Price
LS-C185775-100
100 µl (0.82 mg/ml)
Unavailable

Popular USP47 Products

Detection of Mouse USP47 by Immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-USP47 used at a dilution of 1:250.
Species: Human, Mouse, Bovine, Dog, Pig, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Immunofluorescence
Detection of Human USP47 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-USP47 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
Species: Human, Bovine, Dog, Pig, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Immunofluorescence, Immunoprecipitation
Immunohistochemistry of paraffin-embedded human prostate cancer using antibody at dilution of 1:100.
Species: Human
Applications: IHC, IHC - Paraffin, ELISA
Antibody staining USP47 in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

USP47 antibody LS-C185775 is an unconjugated rabbit polyclonal antibody to USP47 from human and mouse. Validated for ICC and WB.
About USP47
Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizing POLB thereby playing a role in base-excision repair (BER). Acts as a regulator of cell growth and genome integrity. May also indirectly regulates CDC25A expression at a transcriptional level. Q96K76 NM_017944 NP_060414.3

USP47 Antibody, Deubiquitinating enzyme 47 Antibody, Ubiquitin thiolesterase 47 Antibody, Ubiquitin specific protease 47 Antibody, Ubiquitin thioesterase 47 Antibody, TRFP Antibody


Specifications

Target
Human USP47
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Immunogen
USP47 antibody was raised against recombinant protein fragment contain a sequence corresponding to a region within amino acids 635 and 870 of USP47. Percent identity by BLAST analysis: Human (100%); Rat (92%).
Epitope
aa635-870
Specificity
Human USP47
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunocytochemistry

Immunofluorescence of methanol-fixed HeLa using USP47 antibody at 1:200 dilution.
Immunofluorescence of methanol-fixed HeLa using USP47 antibody at 1:200 dilution.

Western blot

Sample (50 ug of whole cell lysate). A: Mouse brain. 5% SDS PAGE. USP47 antibody diluted at 1:1000.
Sample (50 ug of whole cell lysate). A: Mouse brain. 5% SDS PAGE. USP47 antibody diluted at 1:1000.

Western blot

Sample (30 ug of whole cell lysate). A: Hela. 5% SDS PAGE. USP47 antibody. USP47 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Hela. 5% SDS PAGE. USP47 antibody. USP47 antibody diluted at 1:1000.

Immunocytochemistry

Immunofluorescence of methanol-fixed HeLa using USP47 antibody at 1:200 dilution.
Immunofluorescence of methanol-fixed HeLa using USP47 antibody at 1:200 dilution.

Western blot

Sample (50 ug of whole cell lysate). A: Mouse brain. 5% SDS PAGE. USP47 antibody diluted at 1:1000.
Sample (50 ug of whole cell lysate). A: Mouse brain. 5% SDS PAGE. USP47 antibody diluted at 1:1000.

Western blot

Sample (30 ug of whole cell lysate). A: Hela. 5% SDS PAGE. USP47 antibody. USP47 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Hela. 5% SDS PAGE. USP47 antibody. USP47 antibody diluted at 1:1000.

Immunocytochemistry

Immunofluorescence of methanol-fixed HeLa using USP47 antibody at 1:200 dilution.
Immunofluorescence of methanol-fixed HeLa using USP47 antibody at 1:200 dilution.

Western blot

Sample (50 ug of whole cell lysate). A: Mouse brain. 5% SDS PAGE. USP47 antibody diluted at 1:1000.
Sample (50 ug of whole cell lysate). A: Mouse brain. 5% SDS PAGE. USP47 antibody diluted at 1:1000.

Western blot

Sample (30 ug of whole cell lysate). A: Hela. 5% SDS PAGE. USP47 antibody. USP47 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Hela. 5% SDS PAGE. USP47 antibody. USP47 antibody diluted at 1:1000.

Immunocytochemistry

Immunofluorescence of methanol-fixed HeLa using USP47 antibody at 1:200 dilution.
Immunofluorescence of methanol-fixed HeLa using USP47 antibody at 1:200 dilution.

Western blot

Sample (50 ug of whole cell lysate). A: Mouse brain. 5% SDS PAGE. USP47 antibody diluted at 1:1000.
Sample (50 ug of whole cell lysate). A: Mouse brain. 5% SDS PAGE. USP47 antibody diluted at 1:1000.

Western blot

Sample (30 ug of whole cell lysate). A: Hela. 5% SDS PAGE. USP47 antibody. USP47 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Hela. 5% SDS PAGE. USP47 antibody. USP47 antibody diluted at 1:1000.


Requested From: 
Date Requested: 6/21/2018

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