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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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USP33 / VDU1 Antibody (Internal) IHC‑plus™ LS‑A9089 Best Seller
Note: This antibody replaces LS-C91876
VDU1 antibody LS-A9089 is an unconjugated rabbit polyclonal antibody to human VDU1 (USP33). Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A9089-50
50 µg (1 mg/ml)
Unavailable
In Stock for Immediate Shipment

Popular USP33 / VDU1 Products

Anti-USP33 antibody LS-A9090 IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Bat, Bovine, Dog, Horse, Rabbit
Applications: IHC, IHC - Paraffin
Anti-USP33 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5452 concentration 5 ug/ml.
Species: Human
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Detection of Human USP33 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-USP33 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
Species: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Rabbit
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Detection of Human USP33 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-USP33 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
Species: Human, Bovine, Dog, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Immunofluorescence, Immunoprecipitation

Product Description

VDU1 antibody LS-A9089 is an unconjugated rabbit polyclonal antibody to human VDU1 (USP33). Validated for IHC. Tested on 20 paraffin-embedded human tissues.
About USP33 / VDU1
Deubiquitinating enzyme involved in various processes such as centrosome duplication, cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in regulation of centrosome duplication by mediating deubiquitination of CCP110 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Q8TEY7 NM_015017 NP_055832.3

USP33 Antibody, Deubiquitinating enzyme 33 Antibody, KIAA1097 Antibody, HVDU1 Antibody, VDU1 Antibody, Ubiquitin thioesterase 33 Antibody, Ubiquitin thiolesterase 33 Antibody, Ubiquitin specific protease 33 Antibody


Specifications

Target
Human USP33 / VDU1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
USP33 / VDU1 antibody was raised against synthetic 14 amino acid peptide from internal region of human USP33. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Monkey (93%); Orangutan, Gibbon, Marmoset, Dog, Bovine, Panda (86%).
Blocking Peptide
LS-E29556 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A9089. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Internal
Specificity
Human USP33. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: LS-A9089 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A9089 was determined to be 5 ug/ml.
Presentation
PBS, less than 0.1% sodium azide.
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-USP33 antibody LS-A9089 IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-USP33 antibody LS-A9089 IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-USP33 antibody LS-A9089 IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-USP33 antibody LS-A9089 IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-USP33 antibody LS-A9089 IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-USP33 antibody LS-A9089 IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-USP33 antibody LS-A9089 IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-USP33 antibody LS-A9089 IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.


Requested From: 
Date Requested: 6/25/2018

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