Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Yeast (tested or 100% immunogen sequence identity)
Ion exchange chromatography
Western blot (1:500 - 1:2000)
ELISA (1:4000 - 1:20000)
Specificity and Use
Ulp1 antibody was raised against recombinant yeast ULP-1 protein.
anti-Rabbit Serum. Reactivity against ULP-1 from other sources or ULP-2 has not been determined
This purified polyclonal antibody reacts with yeast ULP-1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 72.4 kD in size corresponding to yeast ULP-1 by western blotting in the appropriate lysate or extract.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
0.1 ml deionized water
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
Anti-Yeast ULP-1 Antibody - Western Blot. Western blot of Affinity Purified anti-Yeast ULP-1 antibody was used to confirm the specificity of the antibody. SDS-PAGE of 2 ug of ULP-1 homologues from other sources (lanes 2 through 9). After blocking for 1 hour with 5% non-fat dry milk in TTBS, the blot was probed overnight at 4C with a 1:1000 dilution of anti-yULP1 antibody detected as above. This antibody is specific for yeast ULP1 and does not react with ULP1 from related sources including human SENP.
Anti-Yeast ULP-1 Antibody - Western Blot. Western blot of Affinity Purified anti-Yeast ULP-1 antibody shows detection of a truncated ULP-1 fusion protein (arrowhead). Increasing concentrations of yeast ULP-1 were run on a SDS-PAGE, transferred onto nitrocellulose, and blocked for 1 hour with 5% non-fat dry milk in TTBS, and probed overnight at 4C with a 1:1000 dilution of anti-yULP-1 antibody in 5% non-fat dry milk in TTBS. Detection occurred using a 1:1000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 3-sec exposure time.