Target
Human UHRF1
Synonyms
UHRF1 | HUHRF1 | HuNp95 | Nuclear protein 95 | RING finger protein 106 | RNF106 | Transcription factor ICBP90 | HNP95 | ICBP90 | Np95
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-LNQLFPGYGNGR, from the C-Terminus of protein sequence according to NP_001041666.1NP_037414.3.
Specificity
Human UHRF1. This antibody is expected to recognise both reported isoforms.
Applications
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:2000)
Usage
Peptide ELISA: antibody detection limit dilution 1:2000. Western blot: Approx 100kD band observed in nuclear lysates of cell line Jurkat (calculated MW of 91.1kD according to NP_037414.3). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Hopfner, Cancer Res. 2000 Jan 1;60(1):121-8. PMID: 10646863). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee