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Anti-UBQLN1 / Ubiquilin Antibody (aa290-339) IHC-plus™ LS-B3574

Note: This antibody replaces LS-C81970

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular UBQLN1 / Ubiquilin Antibodies

Anti-UBQLN1 / Ubiquilin Antibody (Internal, clone 3D5E2) IHC-plus™ LS-B3142
Mouse Monoclonal [clone 3D5E2] (IgG2b,k) to Human UBQLN1 / Ubiquilin
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-UBQLN1 / Ubiquilin Antibody (aa290-339) IHC-plus™ LS-B3574
Rabbit Polyclonal (IgG) to Human UBQLN1 / Ubiquilin
Human, Monkey, Bovine, Dog, Guinea pig, Horse, Rabbit, Sheep
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-UBQLN1 / Ubiquilin Antibody (aa19-49) IHC-plus™ LS-B6344
Rabbit Polyclonal to Human UBQLN1 / Ubiquilin
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human UBQLN1 / Ubiquilin
Human, Monkey, Bovine, Dog, Guinea pig, Horse, Rabbit, Sheep
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human UBQLN1 / Ubiquilin
Rabbit
Human, Monkey, Bovine, Dog, Guinea pig, Horse, Rabbit, Sheep (tested or 100% immunogen sequence identity)
Mouse, Rat, Bat (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

UBQLN1 / Ubiquilin antibody was raised against synthetic peptide located between aa290-339 of human UBQLN1 (Q5T6J9, NP_038466). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Sheep, Elephant, Dog, Bovine, Rabbit, Horse, Guinea pig, Platypus (100%); Mouse, Rat, Panda, Bat (92%); Chicken (85%).
LS-E19827 - Lyophilized - 100 µg - $145.00
aa290-339
Human UBQLN1
Immunohistochemistry: LS-B3574 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3574 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About UBQLN1 / Ubiquilin

Q9UMX0 NM_013438 Q9UMX0

UBQLN1 Antibody, DA41 Antibody, HPLIC-1 Antibody, PLIC-1 Antibody, Ubiquilin Antibody, UBQN Antibody, XDRP1 Antibody, DSK2 Antibody, PLIC1 Antibody, Ubiquilin 1 Antibody, Ubiquilin-1 Antibody

UBQLN1 / Ubiquilin is an ubiquitin-like protein (ubiquilin) that shares a high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain an N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation.

Immunohistochemistry

Immunohistochemistry
Anti-UBQLN1 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3574 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-UBQLN1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3574 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-UBQLN1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3574 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/6/2016

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