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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Ubiquitin Antibody LS‑C43546 Best Seller
Note: This antibody replaces LS-C43547
Ubiquitin antibody LS-C43546 is an unconjugated mouse monoclonal antibody to Ubiquitin from human and bovine. Validated for ELISA, Flow, IHC and WB.
Catalog
Size
Price
LS-C43546-100
100 µg (1 mg/ml)
Unavailable

Popular Ubiquitin Products

Formalin-fixed, paraffin-embedded human brain Alzheimer's disease stained with peroxidase-conjugate and AEC. Note staining of senile plaques and neurons.
Species: Human
Applications: IHC, IHC - Paraffin
293T cells were treated with vehicle (1) or proteosome inhibitor (2) for 4 hours. Lysates were then immunoblotted with antibody anti-ubiquitin monoclonal antibody at 2 ug/ml and revealed with anti-mouse HRP.
Species:
Applications: Western blot, Immunoprecipitation
Anti-Ubiquitin antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3695 concentration 10 ug/ml.
Species: Bovine, Human, Mouse, Rat, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Anti-Ub antibody at 1:500 dilution. 293HEK cells transfected lysate at 100 ug per lane. Rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution;
Species: Human, Monkey, Mouse, Rat, Bovine, Cat, Dog, Goat, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Donkey
Applications: Western blot

Product Description

Ubiquitin antibody LS-C43546 is an unconjugated mouse monoclonal antibody to Ubiquitin from human and bovine. Validated for ELISA, Flow, IHC and WB.
About Ubiquitin
Ubiquitin is a small (8.5 kDa) regulatory protein that has been found in almost all tissues (ubiquitously) of eukaryotic organisms. It was discovered in 1975 by Goldstein and further characterized throughout the 1970s and 1980s. There are four genes in the human genome that produce ubiquitin: UBB, UBC, UBA52 and RPS27A. Ubiquitination is a post-translational modification (an addition to a protein after it has been made) where ubiquitin is attached to a substrate protein.

Specifications

Target
Bovine Ubiquitin
Host
Mouse
Reactivity
Bovine, Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:500 - 1:1000)
  • IHC - Frozen (1:500 - 1:1000)
  • Western blot
  • Flow Cytometry (1:10 - 1:50)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Specificity
Recognizes both human and bovine ubiquitin.
Usage
Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Presentation
PBS, pH 7.4, 0.09% sodium azide
Storage
4°C or -20°C, Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Staining of human hypophysis. Formalin-fixed, paraffin processed tissue with Mouse anti-Ubiquitin (LS-C43546).
Staining of human hypophysis. Formalin-fixed, paraffin processed tissue with Mouse anti-Ubiquitin (LS-C43546).

Western blot

GST-ubiquitin detected with Mouse anti-Ubiquitin (LS-C43546).
GST-ubiquitin detected with Mouse anti-Ubiquitin (LS-C43546).

Immunohistochemistry

Staining of human hypophysis. Formalin-fixed, paraffin processed tissue with Mouse anti-Ubiquitin (LS-C43546).
Staining of human hypophysis. Formalin-fixed, paraffin processed tissue with Mouse anti-Ubiquitin (LS-C43546).

Western blot

GST-ubiquitin detected with Mouse anti-Ubiquitin (LS-C43546).
GST-ubiquitin detected with Mouse anti-Ubiquitin (LS-C43546).

Immunohistochemistry

Staining of human hypophysis. Formalin-fixed, paraffin processed tissue with Mouse anti-Ubiquitin (LS-C43546).
Staining of human hypophysis. Formalin-fixed, paraffin processed tissue with Mouse anti-Ubiquitin (LS-C43546).

Western blot

GST-ubiquitin detected with Mouse anti-Ubiquitin (LS-C43546).
GST-ubiquitin detected with Mouse anti-Ubiquitin (LS-C43546).

Immunohistochemistry

Staining of human hypophysis. Formalin-fixed, paraffin processed tissue with Mouse anti-Ubiquitin (LS-C43546).
Staining of human hypophysis. Formalin-fixed, paraffin processed tissue with Mouse anti-Ubiquitin (LS-C43546).

Western blot

GST-ubiquitin detected with Mouse anti-Ubiquitin (LS-C43546).
GST-ubiquitin detected with Mouse anti-Ubiquitin (LS-C43546).


Requested From: 
Date Requested: 6/22/2018

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