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| Catalog Number | Size | Price |
|---|---|---|
| LS-C140202-100 | 100 µg | $554 |
Monoclonal Mouse anti‑Bovine Ubiquitin Antibody (clone Ubi‑1, IHC, IF, WB) LS‑C140202
Monoclonal Mouse anti‑Bovine Ubiquitin Antibody (clone Ubi‑1, IHC, IF, WB) LS‑C140202
Antibody:
Ubiquitin Mouse anti-Bovine Monoclonal (Ubi-1) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, WB, IP
Reactivity:
Bovine
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
Ubiquitin Mouse anti-Bovine Monoclonal (Ubi-1) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, WB, IP
Reactivity:
Bovine
Format:
Unconjugated, Unmodified
Specifications
Description
Ubiquitin antibody LS-C140202 is an unconjugated mouse monoclonal antibody to bovine Ubiquitin. Validated for IF, IHC, IP and WB.
Host
Mouse
Reactivity
Bovine
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
Ubi-1
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Immunogen
Purified bovine ubiquitin conjugated to carrier protein.
Specificity
Ubiquitin
Applications
- IHC
- IHC - Paraffin
- IHC - Frozen
- Immunofluorescence
- Western blot
- Immunoprecipitation
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
For immunohistology, immunofluorescence and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For Western blotting it is recommended to use lysis buffer in 10 mM N-ethylmaleimide (e. g. Pierce #23030) to inhibit ubiquitin-conjugating enzymes. N-ethylmaleimide inactivates certain enzymes by blocking free sulfhydryls. After electrophoresis and transfer, pre-incubate transferred membranes in denaturing buffer (6 M guanidine-HCl, 20 mM Tris-HCl, 5 mM beta-mercaptoethanol, 1 mM PMSF (pH 7.5)) for 30-60 minutes at 4°C, followed by extensive PBS washing.
Presentation
PBS, 0.02% Sodium Azide, 0.1% BSA
Storage
Store at 4°C, stable for 1 year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Bovine
Applications:
IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry, ELISA
Species:
Bovine, Human, Mouse, Rat, Rabbit
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Monkey, Mouse, Rat, Bovine, Cat, Dog, Goat, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Donkey
Applications:
Western blot
Species:
Human, Mouse, Rat
Applications:
Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
Date Requested: 4/25/2024
