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Anti-Ubiquitin Antibody (clone P4G7-H11) IHC-plus™ LS-B3695

Note: This antibody replaces LS-C104863

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Ubiquitin Antibodies

Anti-Ubiquitin Antibody (clone P4D1, Biotin) LS-C106167
Mouse Monoclonal [clone P4D1] (IgG1) to Human Ubiquitin
Human
Western blot
Biotin Conjugated
Western blot Image
Anti-Ubiquitin Antibody (clone P4D1) LS-C107107
Mouse Monoclonal [clone P4D1] (IgG1)
Western blot, Immunoprecipitation
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone P4G7-H11] (IgG1) to Bovine Ubiquitin
Bovine, Human, Mouse, Rat, Rabbit
IHC - Paraffin, Western blot
Unconjugated

Details

Bovine Ubiquitin
Mouse
Bovine, Human, Mouse, Rat, Rabbit (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [P4G7-H11]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000)

Specificity and Use

Ubiquitin antibody was raised against native bovine Ubiquitin.
Immunohistochemistry: LS-B3695 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3695 was determined to be 10 ug/ml

Packaging

PBS, 0.09% azide, 50% glycerol
Store at -20°C.
For research use only.

About Ubiquitin

Ubiquitin is a small (8.5 kDa) regulatory protein that has been found in almost all tissues (ubiquitously) of eukaryotic organisms. It was discovered in 1975 by Goldstein and further characterized throughout the 1970s and 1980s. There are four genes in the human genome that produce ubiquitin: UBB, UBC, UBA52 and RPS27A. Ubiquitination is a post-translational modification (an addition to a protein after it has been made) where ubiquitin is attached to a substrate protein.

Immunohistochemistry

Immunohistochemistry
Anti-Ubiquitin antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3695 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-Ubiquitin antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3695 concentration 10 ug/ml.

Western blot

Western blot
Western blot of Ubiquitin: Lane 1: MWM, Lane 2: Ubiquitin, Lane 3: HeLa, Lane 4: RK-13, Lane 5:3T3, Lane 6: PC-12, Lane 7: Mouse Brain, Lane 8: Rat Brain, Lane 9: MDBK.
 

Requested From: United States
Date Requested: 12/10/2016

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