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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Ubiquitin Antibody (clone P4G7‑H11) IHC‑plus™ LS‑B3695
Note: This antibody replaces LS-C104863
Ubiquitin antibody LS-B3695 is an unconjugated mouse monoclonal antibody to Ubiquitin from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B3695-50
50 µg (1 mg/ml)
Unavailable

Popular Ubiquitin Products

Staining of human hypophysis. Formalin-fixed, paraffin processed tissue with Mouse anti-Ubiquitin (LS-C43546).
Species: Bovine, Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry, ELISA
Formalin-fixed, paraffin-embedded human brain Alzheimer's disease stained with peroxidase-conjugate and AEC. Note staining of senile plaques and neurons.
Species: Human
Applications: IHC, IHC - Paraffin
293T cells were treated with vehicle (1) or proteosome inhibitor (2) for 4 hours. Lysates were then immunoblotted with antibody anti-ubiquitin monoclonal antibody at 2 ug/ml and revealed with anti-mouse HRP.
Species:
Applications: Western blot, Immunoprecipitation
Anti-Ub antibody at 1:500 dilution. 293HEK cells transfected lysate at 100 ug per lane. Rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution;
Species: Human, Monkey, Mouse, Rat, Bovine, Cat, Dog, Goat, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Donkey
Applications: Western blot

Product Description

Ubiquitin antibody LS-B3695 is an unconjugated mouse monoclonal antibody to Ubiquitin from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About Ubiquitin
Ubiquitin is a small (8.5 kDa) regulatory protein that has been found in almost all tissues (ubiquitously) of eukaryotic organisms. It was discovered in 1975 by Goldstein and further characterized throughout the 1970s and 1980s. There are four genes in the human genome that produce ubiquitin: UBB, UBC, UBA52 and RPS27A. Ubiquitination is a post-translational modification (an addition to a protein after it has been made) where ubiquitin is attached to a substrate protein.

Specifications

Target
Bovine Ubiquitin
Host
Mouse
Reactivity
Bovine, Human, Mouse, Rat, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [P4G7-H11]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Ubiquitin antibody was raised against native bovine Ubiquitin.
Usage
Immunohistochemistry: LS-B3695 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3695 was determined to be 10 ug/ml
Presentation
PBS, 0.09% azide, 50% glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-Ubiquitin antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3695 concentration 10 ug/ml.
Anti-Ubiquitin antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3695 concentration 10 ug/ml.

Immunohistochemistry

Anti-Ubiquitin antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3695 concentration 10 ug/ml.
Anti-Ubiquitin antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3695 concentration 10 ug/ml.

Western blot

Western blot of Ubiquitin: Lane 1: MWM, Lane 2: Ubiquitin, Lane 3: HeLa, Lane 4: RK-13, Lane 5:3T3, Lane 6: PC-12, Lane 7: Mouse Brain, Lane 8: Rat Brain, Lane 9: MDBK.
Western blot of Ubiquitin: Lane 1: MWM, Lane 2: Ubiquitin, Lane 3: HeLa, Lane 4: RK-13, Lane 5:3T3, Lane 6: PC-12, Lane 7: Mouse Brain, Lane 8: Rat Brain, Lane 9: MDBK.

Immunohistochemistry

Anti-Ubiquitin antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3695 concentration 10 ug/ml.
Anti-Ubiquitin antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3695 concentration 10 ug/ml.

Immunohistochemistry

Anti-Ubiquitin antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3695 concentration 10 ug/ml.
Anti-Ubiquitin antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3695 concentration 10 ug/ml.

Western blot

Western blot of Ubiquitin: Lane 1: MWM, Lane 2: Ubiquitin, Lane 3: HeLa, Lane 4: RK-13, Lane 5:3T3, Lane 6: PC-12, Lane 7: Mouse Brain, Lane 8: Rat Brain, Lane 9: MDBK.
Western blot of Ubiquitin: Lane 1: MWM, Lane 2: Ubiquitin, Lane 3: HeLa, Lane 4: RK-13, Lane 5:3T3, Lane 6: PC-12, Lane 7: Mouse Brain, Lane 8: Rat Brain, Lane 9: MDBK.

Immunohistochemistry

Anti-Ubiquitin antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3695 concentration 10 ug/ml.
Anti-Ubiquitin antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3695 concentration 10 ug/ml.

Immunohistochemistry

Anti-Ubiquitin antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3695 concentration 10 ug/ml.
Anti-Ubiquitin antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3695 concentration 10 ug/ml.

Western blot

Western blot of Ubiquitin: Lane 1: MWM, Lane 2: Ubiquitin, Lane 3: HeLa, Lane 4: RK-13, Lane 5:3T3, Lane 6: PC-12, Lane 7: Mouse Brain, Lane 8: Rat Brain, Lane 9: MDBK.
Western blot of Ubiquitin: Lane 1: MWM, Lane 2: Ubiquitin, Lane 3: HeLa, Lane 4: RK-13, Lane 5:3T3, Lane 6: PC-12, Lane 7: Mouse Brain, Lane 8: Rat Brain, Lane 9: MDBK.

Immunohistochemistry

Anti-Ubiquitin antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3695 concentration 10 ug/ml.
Anti-Ubiquitin antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3695 concentration 10 ug/ml.

Immunohistochemistry

Anti-Ubiquitin antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3695 concentration 10 ug/ml.
Anti-Ubiquitin antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3695 concentration 10 ug/ml.

Western blot

Western blot of Ubiquitin: Lane 1: MWM, Lane 2: Ubiquitin, Lane 3: HeLa, Lane 4: RK-13, Lane 5:3T3, Lane 6: PC-12, Lane 7: Mouse Brain, Lane 8: Rat Brain, Lane 9: MDBK.
Western blot of Ubiquitin: Lane 1: MWM, Lane 2: Ubiquitin, Lane 3: HeLa, Lane 4: RK-13, Lane 5:3T3, Lane 6: PC-12, Lane 7: Mouse Brain, Lane 8: Rat Brain, Lane 9: MDBK.


Requested From: 
Date Requested: 6/22/2018

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